Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24000 | 3' | -53.3 | NC_005262.1 | + | 45012 | 0.66 | 0.85607 |
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Target: 5'- gGGGCuGUCGUAGuUCUUCgCCgugUCg -3' miRNA: 3'- gCCCGcCAGCGUUcAGAAG-GGaaaAG- -5' |
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| 24000 | 3' | -53.3 | NC_005262.1 | + | 23634 | 0.66 | 0.847564 |
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Target: 5'- gCGGGCGGUuggucagucugCGCG-GUCcUCCCgcgcUUCc -3' miRNA: 3'- -GCCCGCCA-----------GCGUuCAGaAGGGaa--AAG- -5' |
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| 24000 | 3' | -53.3 | NC_005262.1 | + | 62546 | 0.67 | 0.81135 |
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Target: 5'- uGGGCGGUUGUggGgaucacgUCCUgg-UCg -3' miRNA: 3'- gCCCGCCAGCGuuCaga----AGGGaaaAG- -5' |
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| 24000 | 3' | -53.3 | NC_005262.1 | + | 22383 | 0.67 | 0.801796 |
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Target: 5'- cCGGcGCGGaUCGCGA-UCaUCCCgcgUUCg -3' miRNA: 3'- -GCC-CGCC-AGCGUUcAGaAGGGaa-AAG- -5' |
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| 24000 | 3' | -53.3 | NC_005262.1 | + | 44752 | 0.68 | 0.751559 |
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Target: 5'- gCGGGCGcGUCGCcggcGUCUUUCUUg--- -3' miRNA: 3'- -GCCCGC-CAGCGuu--CAGAAGGGAaaag -5' |
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| 24000 | 3' | -53.3 | NC_005262.1 | + | 4410 | 0.72 | 0.52318 |
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Target: 5'- aCGGGCGGUCGUcGGcuUCUUUUCUgggcUUCa -3' miRNA: 3'- -GCCCGCCAGCGuUC--AGAAGGGAa---AAG- -5' |
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| 24000 | 3' | -53.3 | NC_005262.1 | + | 51096 | 0.72 | 0.52318 |
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Target: 5'- aCGGcGCGGUCGCcGGUCgcUUCCUUg-- -3' miRNA: 3'- -GCC-CGCCAGCGuUCAGa-AGGGAAaag -5' |
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| 24000 | 3' | -53.3 | NC_005262.1 | + | 19807 | 1.09 | 0.001922 |
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Target: 5'- uCGGGCGGUCGCAAGUCUUCCCUUUUCc -3' miRNA: 3'- -GCCCGCCAGCGUUCAGAAGGGAAAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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