Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24002 | 3' | -53.3 | NC_005262.1 | + | 35199 | 0.66 | 0.844755 |
Target: 5'- ----cGCGUGGCCAgccuacGUCCGCaaggAUGCGg -3' miRNA: 3'- gacaaCGCAUCGGU------UAGGCGg---UGUGC- -5' |
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24002 | 3' | -53.3 | NC_005262.1 | + | 21681 | 0.66 | 0.844755 |
Target: 5'- cCUGagcgGCGgcGCCGcgCUGCUguACGCGg -3' miRNA: 3'- -GACaa--CGCauCGGUuaGGCGG--UGUGC- -5' |
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24002 | 3' | -53.3 | NC_005262.1 | + | 54424 | 0.66 | 0.8269 |
Target: 5'- ----cGCGgaGGCgCAggCCGCCGCGCGc -3' miRNA: 3'- gacaaCGCa-UCG-GUuaGGCGGUGUGC- -5' |
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24002 | 3' | -53.3 | NC_005262.1 | + | 49155 | 0.66 | 0.861707 |
Target: 5'- -gGUggGCGUgcaGGCCGcAUCgGCCGCAgCGg -3' miRNA: 3'- gaCAa-CGCA---UCGGU-UAGgCGGUGU-GC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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