miRNA: 3'- gaCAACGCauCGGUUAGGCGG------------UGugC- -5'
miRNA: 3'- gacaaCGCa-UCG-GUuaGGCGGUGUGC- -5'
miRNA: 3'- gacaaCGCa---UCGGUUAGGCGG---UGUGC- -5'
miRNA: 3'- gACaACGCauCGGUUaGGCGGU-GUGC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24002 | 3' | -53.3 | NC_005262.1 | + | 51584 | 0.67 | 0.76869 |
|
Target: 5'- -cGUUGCG--GCCGAUCCGCUcgaucucguaggACugGc -3' miRNA: 3'- gaCAACGCauCGGUUAGGCGG------------UGugC- -5' |
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| 24002 | 3' | -53.3 | NC_005262.1 | + | 54424 | 0.66 | 0.8269 |
|
Target: 5'- ----cGCGgaGGCgCAggCCGCCGCGCGc -3' miRNA: 3'- gacaaCGCa-UCG-GUuaGGCGGUGUGC- -5' |
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| 24002 | 3' | -53.3 | NC_005262.1 | + | 59214 | 0.68 | 0.748027 |
|
Target: 5'- ----cGCGgcccGGCCGGUUCGCCucgGCGCGg -3' miRNA: 3'- gacaaCGCa---UCGGUUAGGCGG---UGUGC- -5' |
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| 24002 | 3' | -53.3 | NC_005262.1 | + | 62828 | 0.67 | 0.778813 |
|
Target: 5'- aUGcUGCGgcGCUGA-CCGCCAgCGCGc -3' miRNA: 3'- gACaACGCauCGGUUaGGCGGU-GUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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