Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24011 | 3' | -54.4 | NC_005262.1 | + | 7728 | 0.66 | 0.743694 |
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Target: 5'- cGCUGaccgcucaGCGuGAGGCUGAGCUGggaaUGUUg -3' miRNA: 3'- uUGACg-------UGC-UUCCGGCUCGACa---ACAA- -5' |
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| 24011 | 3' | -54.4 | NC_005262.1 | + | 27646 | 0.66 | 0.743694 |
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Target: 5'- cGCUGCGCGGcguccucgccgGGGCUGAGCUc----- -3' miRNA: 3'- uUGACGUGCU-----------UCCGGCUCGAcaacaa -5' |
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| 24011 | 3' | -54.4 | NC_005262.1 | + | 32863 | 0.66 | 0.732928 |
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Target: 5'- ---cGCACGugcGGcGCCGAGCUGUUc-- -3' miRNA: 3'- uugaCGUGCu--UC-CGGCUCGACAAcaa -5' |
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| 24011 | 3' | -54.4 | NC_005262.1 | + | 27604 | 0.66 | 0.700044 |
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Target: 5'- --gUGCGCGcGGGCaCGAGCUGggugcUGUc -3' miRNA: 3'- uugACGUGCuUCCG-GCUCGACa----ACAa -5' |
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| 24011 | 3' | -54.4 | NC_005262.1 | + | 25457 | 0.67 | 0.65528 |
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Target: 5'- -cCUGCGCGAugauGCCGcaccGGCUGUUGg- -3' miRNA: 3'- uuGACGUGCUuc--CGGC----UCGACAACaa -5' |
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| 24011 | 3' | -54.4 | NC_005262.1 | + | 11683 | 0.68 | 0.6203 |
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Target: 5'- gAGCUucGCGCGcaacuucggaaccGAGGCCGAGCUGcUGc- -3' miRNA: 3'- -UUGA--CGUGC-------------UUCCGGCUCGACaACaa -5' |
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| 24011 | 3' | -54.4 | NC_005262.1 | + | 57366 | 0.72 | 0.399452 |
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Target: 5'- uGCUGCACGcGGGCCgugagcugcgcGAGCUGgUUGUc -3' miRNA: 3'- uUGACGUGCuUCCGG-----------CUCGAC-AACAa -5' |
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| 24011 | 3' | -54.4 | NC_005262.1 | + | 6367 | 0.73 | 0.32081 |
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Target: 5'- ---gGCgGCGAAGGCCGAGCUGcUGa- -3' miRNA: 3'- uugaCG-UGCUUCCGGCUCGACaACaa -5' |
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| 24011 | 3' | -54.4 | NC_005262.1 | + | 25486 | 1.02 | 0.002936 |
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Target: 5'- cAACUGCACGAAGGCCGAGCUGUUGUUc -3' miRNA: 3'- -UUGACGUGCUUCCGGCUCGACAACAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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