Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24013 | 3' | -54.7 | NC_005262.1 | + | 53585 | 0.66 | 0.785498 |
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Target: 5'- cCCCUGCggcGUCUgcacUUCGGcCGUGCCg -3' miRNA: 3'- aGGGACGga-CAGGac--AAGUU-GCACGG- -5' |
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| 24013 | 3' | -54.7 | NC_005262.1 | + | 56930 | 0.66 | 0.765539 |
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Target: 5'- -gCCggaGCUUGUUCUGgucgUCGugGUGCg -3' miRNA: 3'- agGGa--CGGACAGGACa---AGUugCACGg -5' |
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| 24013 | 3' | -54.7 | NC_005262.1 | + | 43718 | 0.67 | 0.745037 |
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Target: 5'- cUCCUGCCaGUUCUGcgCcg-GUGCCg -3' miRNA: 3'- aGGGACGGaCAGGACaaGuugCACGG- -5' |
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| 24013 | 3' | -54.7 | NC_005262.1 | + | 23734 | 0.67 | 0.724082 |
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Target: 5'- aCCCUGCUUugaccaaCCUGcccgCAGCGUGCg -3' miRNA: 3'- aGGGACGGAca-----GGACaa--GUUGCACGg -5' |
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| 24013 | 3' | -54.7 | NC_005262.1 | + | 9213 | 0.67 | 0.701691 |
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Target: 5'- gCCCggcGCCgGUCCUcauagccgcugcgGUUCAGCGUucccgaGCCu -3' miRNA: 3'- aGGGa--CGGaCAGGA-------------CAAGUUGCA------CGG- -5' |
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| 24013 | 3' | -54.7 | NC_005262.1 | + | 13832 | 0.68 | 0.681175 |
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Target: 5'- aCCCgcgagGCUgaaugCCUGgcaCGGCGUGCCa -3' miRNA: 3'- aGGGa----CGGaca--GGACaa-GUUGCACGG- -5' |
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| 24013 | 3' | -54.7 | NC_005262.1 | + | 51169 | 0.68 | 0.637525 |
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Target: 5'- gUCCUUGCCUuccUCCUGcgCGGCGagcaccugauggUGCCc -3' miRNA: 3'- -AGGGACGGAc--AGGACaaGUUGC------------ACGG- -5' |
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| 24013 | 3' | -54.7 | NC_005262.1 | + | 44547 | 0.7 | 0.561296 |
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Target: 5'- cUCCUGCCguUCCUGUUCu-CGccgGCCg -3' miRNA: 3'- aGGGACGGacAGGACAAGuuGCa--CGG- -5' |
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| 24013 | 3' | -54.7 | NC_005262.1 | + | 54439 | 0.72 | 0.438111 |
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Target: 5'- gCCCUucGCCggGUCCUcGUagaUCGAgGUGCCg -3' miRNA: 3'- aGGGA--CGGa-CAGGA-CA---AGUUgCACGG- -5' |
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| 24013 | 3' | -54.7 | NC_005262.1 | + | 26079 | 1.13 | 0.00069 |
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Target: 5'- gUCCCUGCCUGUCCUGUUCAACGUGCCg -3' miRNA: 3'- -AGGGACGGACAGGACAAGUUGCACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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