Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24026 | 5' | -56.1 | NC_005262.1 | + | 49192 | 0.66 | 0.692422 |
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Target: 5'- aCGGCGaugauCUCCGGCAugCUGgccGAGGGc -3' miRNA: 3'- -GCCGU-----GGGGUCGUugGAUacaCUCCU- -5' |
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| 24026 | 5' | -56.1 | NC_005262.1 | + | 27256 | 0.66 | 0.670826 |
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Target: 5'- aCGGCACCacaCCAuGCGGCCgGUGUGccGGc -3' miRNA: 3'- -GCCGUGG---GGU-CGUUGGaUACACucCU- -5' |
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| 24026 | 5' | -56.1 | NC_005262.1 | + | 12550 | 0.68 | 0.583807 |
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Target: 5'- gCGGCGCCCCgugAGCGACgUcucgacGAGGAu -3' miRNA: 3'- -GCCGUGGGG---UCGUUGgAuaca--CUCCU- -5' |
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| 24026 | 5' | -56.1 | NC_005262.1 | + | 37119 | 0.68 | 0.55158 |
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Target: 5'- uCGGCACCUuucgcgauCAGCcGCCUGuUGUGGccGGAu -3' miRNA: 3'- -GCCGUGGG--------GUCGuUGGAU-ACACU--CCU- -5' |
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| 24026 | 5' | -56.1 | NC_005262.1 | + | 34533 | 0.69 | 0.509515 |
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Target: 5'- gGGCAgCCCggagcgaacGGCAgaACCUucGUGAGGAa -3' miRNA: 3'- gCCGUgGGG---------UCGU--UGGAuaCACUCCU- -5' |
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| 24026 | 5' | -56.1 | NC_005262.1 | + | 36289 | 0.7 | 0.47887 |
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Target: 5'- gCGGCgacgcgaacACCUCGGuCGACUUcgGUGGGGGg -3' miRNA: 3'- -GCCG---------UGGGGUC-GUUGGAuaCACUCCU- -5' |
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| 24026 | 5' | -56.1 | NC_005262.1 | + | 51374 | 0.71 | 0.384044 |
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Target: 5'- gCGGCAUgCgCAGCAGCCggcggccgGUGAGGu -3' miRNA: 3'- -GCCGUGgG-GUCGUUGGaua-----CACUCCu -5' |
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| 24026 | 5' | -56.1 | NC_005262.1 | + | 22776 | 0.76 | 0.206241 |
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Target: 5'- gCGGCACCCCGGCAuuCCcGUccGAGGGg -3' miRNA: 3'- -GCCGUGGGGUCGUu-GGaUAcaCUCCU- -5' |
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| 24026 | 5' | -56.1 | NC_005262.1 | + | 29797 | 1.09 | 0.000988 |
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Target: 5'- gCGGCACCCCAGCAACCUAUGUGAGGAc -3' miRNA: 3'- -GCCGUGGGGUCGUUGGAUACACUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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