miRNA: 3'- -GAgCUGC-UGGa------CUUGuUCCGGCUCGc -5'
miRNA: 3'- gaGCUGCUGga-CUugUUCCGGCUCgC- -5'
miRNA: 3'- gAGCUGCUGgACU--------------UGUuCCGGCu-CGC- -5'
miRNA: 3'- -GAGCUGCUGGACu---UGUuCCGGcUCGC- -5'
miRNA: 3'- -GAGCUGCUGGa-CUUGUUCCGgCuC-GC- -5'
Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24027 | 3' | -55.2 | NC_005262.1 | + | 57337 | 0.66 | 0.770762 |
|
Target: 5'- uCUgGGCGcGCCguucccgGAAC-AGGCUGAGCu -3' miRNA: 3'- -GAgCUGC-UGGa------CUUGuUCCGGCUCGc -5' |
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| 24027 | 3' | -55.2 | NC_005262.1 | + | 57461 | 0.66 | 0.780635 |
|
Target: 5'- -gCGGCGGCgacGAugGAGGUCGAGgGc -3' miRNA: 3'- gaGCUGCUGga-CUugUUCCGGCUCgC- -5' |
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| 24027 | 3' | -55.2 | NC_005262.1 | + | 58801 | 0.66 | 0.757721 |
|
Target: 5'- gUCGACGACgUGAucggcgccgugaagGCAuGGCUGcuGCGg -3' miRNA: 3'- gAGCUGCUGgACU--------------UGUuCCGGCu-CGC- -5' |
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| 24027 | 3' | -55.2 | NC_005262.1 | + | 60593 | 0.71 | 0.465826 |
|
Target: 5'- uCUUGGCGGCCUGcgccGCGcGGCCcGGCGu -3' miRNA: 3'- -GAGCUGCUGGACu---UGUuCCGGcUCGC- -5' |
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| 24027 | 3' | -55.2 | NC_005262.1 | + | 61556 | 0.73 | 0.399639 |
|
Target: 5'- uCUCGGCGAUCcgGGACGAGGCgGuGaCGg -3' miRNA: 3'- -GAGCUGCUGGa-CUUGUUCCGgCuC-GC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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