Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24027 | 3' | -55.2 | NC_005262.1 | + | 26349 | 0.67 | 0.716382 |
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Target: 5'- gUCGuGCGGCagGAACAucgugucccacacgAGGCCGAGCc -3' miRNA: 3'- gAGC-UGCUGgaCUUGU--------------UCCGGCUCGc -5' |
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| 24027 | 3' | -55.2 | NC_005262.1 | + | 55258 | 0.67 | 0.708995 |
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Target: 5'- gCUCGuccGCGACCaUGAuguuCGcGGCCGGGUa -3' miRNA: 3'- -GAGC---UGCUGG-ACUu---GUuCCGGCUCGc -5' |
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| 24027 | 3' | -55.2 | NC_005262.1 | + | 48692 | 0.67 | 0.698381 |
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Target: 5'- cCUCGuCGAgCUGcgauuGC-AGGCCGAGgCGg -3' miRNA: 3'- -GAGCuGCUgGACu----UGuUCCGGCUC-GC- -5' |
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| 24027 | 3' | -55.2 | NC_005262.1 | + | 29260 | 0.67 | 0.698381 |
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Target: 5'- gCUCGGCGacggccGCCUGAcGCAuGGUCGcGCGc -3' miRNA: 3'- -GAGCUGC------UGGACU-UGUuCCGGCuCGC- -5' |
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| 24027 | 3' | -55.2 | NC_005262.1 | + | 20845 | 0.65 | 0.796117 |
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Target: 5'- uCUCGuggcgcugggguuCGACC-GGGCAGGggucgagcuGCCGAGCGa -3' miRNA: 3'- -GAGCu------------GCUGGaCUUGUUC---------CGGCUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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