Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24028 | 3' | -57.9 | NC_005262.1 | + | 17072 | 0.66 | 0.593537 |
Target: 5'- cGGCGccgauAGGCGCGCCCguCGAc-GCGGCGg -3' miRNA: 3'- cUCGU-----UCCGCGUGGG--GCUcaUGUCGU- -5' |
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24028 | 3' | -57.9 | NC_005262.1 | + | 13033 | 0.66 | 0.593537 |
Target: 5'- aGGGCuacgcGGUGCGCgUCGAGcUGCGGCc -3' miRNA: 3'- -CUCGuu---CCGCGUGgGGCUC-AUGUCGu -5' |
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24028 | 3' | -57.9 | NC_005262.1 | + | 18956 | 0.66 | 0.582747 |
Target: 5'- -cGCAAGGCGCugCuCCGGaUGC-GCu -3' miRNA: 3'- cuCGUUCCGCGugG-GGCUcAUGuCGu -5' |
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24028 | 3' | -57.9 | NC_005262.1 | + | 4030 | 0.66 | 0.582747 |
Target: 5'- -uGCAcGGUGCugCCUGAGUAacaugacaaaAGCGa -3' miRNA: 3'- cuCGUuCCGCGugGGGCUCAUg---------UCGU- -5' |
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24028 | 3' | -57.9 | NC_005262.1 | + | 9798 | 0.66 | 0.571997 |
Target: 5'- -cGCGuauGGCGC-CCUCGGGUuuGGCGc -3' miRNA: 3'- cuCGUu--CCGCGuGGGGCUCAugUCGU- -5' |
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24028 | 3' | -57.9 | NC_005262.1 | + | 54203 | 0.66 | 0.570925 |
Target: 5'- aGGCGAGG-GCGCCgucgacgUCGAGgcGCAGCAg -3' miRNA: 3'- cUCGUUCCgCGUGG-------GGCUCa-UGUCGU- -5' |
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24028 | 3' | -57.9 | NC_005262.1 | + | 48472 | 0.66 | 0.561296 |
Target: 5'- cAGCGAGGCGCGgucguaaCCCGAcugAUAGCc -3' miRNA: 3'- cUCGUUCCGCGUg------GGGCUca-UGUCGu -5' |
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24028 | 3' | -57.9 | NC_005262.1 | + | 13813 | 0.66 | 0.550649 |
Target: 5'- uGGCAcGGCGUGCCaCCGAc--CAGCAc -3' miRNA: 3'- cUCGUuCCGCGUGG-GGCUcauGUCGU- -5' |
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24028 | 3' | -57.9 | NC_005262.1 | + | 49844 | 0.67 | 0.540066 |
Target: 5'- gGAGCcucGGGCGCgggcGCCgCCGgcgucGGUGCGGCu -3' miRNA: 3'- -CUCGu--UCCGCG----UGG-GGC-----UCAUGUCGu -5' |
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24028 | 3' | -57.9 | NC_005262.1 | + | 53537 | 0.67 | 0.540066 |
Target: 5'- cGAGUAuuucgccGCGUACCCCGAGaUGguGCu -3' miRNA: 3'- -CUCGUuc-----CGCGUGGGGCUC-AUguCGu -5' |
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24028 | 3' | -57.9 | NC_005262.1 | + | 20920 | 0.67 | 0.539011 |
Target: 5'- aGAGCGGGuGcCGCGCCCCGcgauGUucccugucgauucGCGGCu -3' miRNA: 3'- -CUCGUUC-C-GCGUGGGGCu---CA-------------UGUCGu -5' |
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24028 | 3' | -57.9 | NC_005262.1 | + | 45677 | 0.67 | 0.536904 |
Target: 5'- cGGUGAGGCGguCCCaaaacggccgcaucaGGUACAGCAg -3' miRNA: 3'- cUCGUUCCGCguGGGgc-------------UCAUGUCGU- -5' |
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24028 | 3' | -57.9 | NC_005262.1 | + | 13191 | 0.67 | 0.529552 |
Target: 5'- cGGUAAGGCuaACCCUGGuccGUGCGcGCAa -3' miRNA: 3'- cUCGUUCCGcgUGGGGCU---CAUGU-CGU- -5' |
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24028 | 3' | -57.9 | NC_005262.1 | + | 44958 | 0.67 | 0.529552 |
Target: 5'- gGAGCAGauGGC-CGCCgaggcgagggCCGAcGUGCAGCAg -3' miRNA: 3'- -CUCGUU--CCGcGUGG----------GGCU-CAUGUCGU- -5' |
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24028 | 3' | -57.9 | NC_005262.1 | + | 11017 | 0.67 | 0.507727 |
Target: 5'- cGAGCGAGGCggcugucGCGCaagaCCGcGcGCAGCAa -3' miRNA: 3'- -CUCGUUCCG-------CGUGg---GGCuCaUGUCGU- -5' |
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24028 | 3' | -57.9 | NC_005262.1 | + | 15581 | 0.67 | 0.507727 |
Target: 5'- gGAGCugGAGGCGCGCgCgCgCGAggagcgcGUGCGGCAg -3' miRNA: 3'- -CUCG--UUCCGCGUG-G-G-GCU-------CAUGUCGU- -5' |
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24028 | 3' | -57.9 | NC_005262.1 | + | 28044 | 0.67 | 0.49849 |
Target: 5'- aGAGCu-GGCGCAacaucCUCUGGG-ACAGCAc -3' miRNA: 3'- -CUCGuuCCGCGU-----GGGGCUCaUGUCGU- -5' |
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24028 | 3' | -57.9 | NC_005262.1 | + | 15477 | 0.67 | 0.488316 |
Target: 5'- aAGCAcGGCGCuuaCaCCGAGcGCGGCGg -3' miRNA: 3'- cUCGUuCCGCGug-G-GGCUCaUGUCGU- -5' |
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24028 | 3' | -57.9 | NC_005262.1 | + | 28517 | 0.69 | 0.423847 |
Target: 5'- cGAGCAcGGCGCGgCCCGAuucgaccucgagcacGUGCcGCu -3' miRNA: 3'- -CUCGUuCCGCGUgGGGCU---------------CAUGuCGu -5' |
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24028 | 3' | -57.9 | NC_005262.1 | + | 23929 | 0.69 | 0.420104 |
Target: 5'- cGGCAAGGCGU-UUCUGGGUcCAGCAa -3' miRNA: 3'- cUCGUUCCGCGuGGGGCUCAuGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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