Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24028 | 3' | -57.9 | NC_005262.1 | + | 9435 | 0.69 | 0.401694 |
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Target: 5'- cGAGC-GGGUGCGaagCCCGcauuGUGCGGCAc -3' miRNA: 3'- -CUCGuUCCGCGUg--GGGCu---CAUGUCGU- -5' |
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| 24028 | 3' | -57.9 | NC_005262.1 | + | 50351 | 0.7 | 0.325485 |
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Target: 5'- cGGCGcGGGCGCGCCCuCGu-UGCGGCGg -3' miRNA: 3'- cUCGU-UCCGCGUGGG-GCucAUGUCGU- -5' |
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| 24028 | 3' | -57.9 | NC_005262.1 | + | 58504 | 0.71 | 0.317711 |
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Target: 5'- cGGGCAGGGaCGCgGCCgUGucGUGCAGCAc -3' miRNA: 3'- -CUCGUUCC-GCG-UGGgGCu-CAUGUCGU- -5' |
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| 24028 | 3' | -57.9 | NC_005262.1 | + | 35253 | 0.71 | 0.288019 |
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Target: 5'- cGGCuGGGCGC-CCCCGGcGaACGGCAc -3' miRNA: 3'- cUCGuUCCGCGuGGGGCU-CaUGUCGU- -5' |
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| 24028 | 3' | -57.9 | NC_005262.1 | + | 54472 | 0.73 | 0.229268 |
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Target: 5'- -cGuCGAGGCGCugCUCGcGGUGCAGCc -3' miRNA: 3'- cuC-GUUCCGCGugGGGC-UCAUGUCGu -5' |
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| 24028 | 3' | -57.9 | NC_005262.1 | + | 61787 | 0.74 | 0.180967 |
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Target: 5'- cGAGCGAacccGGCGCGCCCUGAucGUcACGGCu -3' miRNA: 3'- -CUCGUU----CCGCGUGGGGCU--CA-UGUCGu -5' |
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| 24028 | 3' | -57.9 | NC_005262.1 | + | 30383 | 1.08 | 0.000648 |
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Target: 5'- cGAGCAAGGCGCACCCCGAGUACAGCAc -3' miRNA: 3'- -CUCGUUCCGCGUGGGGCUCAUGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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