Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24028 | 3' | -57.9 | NC_005262.1 | + | 49844 | 0.67 | 0.540066 |
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Target: 5'- gGAGCcucGGGCGCgggcGCCgCCGgcgucGGUGCGGCu -3' miRNA: 3'- -CUCGu--UCCGCG----UGG-GGC-----UCAUGUCGu -5' |
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| 24028 | 3' | -57.9 | NC_005262.1 | + | 13813 | 0.66 | 0.550649 |
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Target: 5'- uGGCAcGGCGUGCCaCCGAc--CAGCAc -3' miRNA: 3'- cUCGUuCCGCGUGG-GGCUcauGUCGU- -5' |
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| 24028 | 3' | -57.9 | NC_005262.1 | + | 48472 | 0.66 | 0.561296 |
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Target: 5'- cAGCGAGGCGCGgucguaaCCCGAcugAUAGCc -3' miRNA: 3'- cUCGUUCCGCGUg------GGGCUca-UGUCGu -5' |
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| 24028 | 3' | -57.9 | NC_005262.1 | + | 54203 | 0.66 | 0.570925 |
|
Target: 5'- aGGCGAGG-GCGCCgucgacgUCGAGgcGCAGCAg -3' miRNA: 3'- cUCGUUCCgCGUGG-------GGCUCa-UGUCGU- -5' |
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| 24028 | 3' | -57.9 | NC_005262.1 | + | 9798 | 0.66 | 0.571997 |
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Target: 5'- -cGCGuauGGCGC-CCUCGGGUuuGGCGc -3' miRNA: 3'- cuCGUu--CCGCGuGGGGCUCAugUCGU- -5' |
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| 24028 | 3' | -57.9 | NC_005262.1 | + | 18956 | 0.66 | 0.582747 |
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Target: 5'- -cGCAAGGCGCugCuCCGGaUGC-GCu -3' miRNA: 3'- cuCGUUCCGCGugG-GGCUcAUGuCGu -5' |
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| 24028 | 3' | -57.9 | NC_005262.1 | + | 61787 | 0.74 | 0.180967 |
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Target: 5'- cGAGCGAacccGGCGCGCCCUGAucGUcACGGCu -3' miRNA: 3'- -CUCGUU----CCGCGUGGGGCU--CA-UGUCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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