Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24028 | 3' | -57.9 | NC_005262.1 | + | 28044 | 0.67 | 0.49849 |
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Target: 5'- aGAGCu-GGCGCAacaucCUCUGGG-ACAGCAc -3' miRNA: 3'- -CUCGuuCCGCGU-----GGGGCUCaUGUCGU- -5' |
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| 24028 | 3' | -57.9 | NC_005262.1 | + | 11017 | 0.67 | 0.507727 |
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Target: 5'- cGAGCGAGGCggcugucGCGCaagaCCGcGcGCAGCAa -3' miRNA: 3'- -CUCGUUCCG-------CGUGg---GGCuCaUGUCGU- -5' |
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| 24028 | 3' | -57.9 | NC_005262.1 | + | 15581 | 0.67 | 0.507727 |
|
Target: 5'- gGAGCugGAGGCGCGCgCgCgCGAggagcgcGUGCGGCAg -3' miRNA: 3'- -CUCG--UUCCGCGUG-G-G-GCU-------CAUGUCGU- -5' |
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| 24028 | 3' | -57.9 | NC_005262.1 | + | 13191 | 0.67 | 0.529552 |
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Target: 5'- cGGUAAGGCuaACCCUGGuccGUGCGcGCAa -3' miRNA: 3'- cUCGUUCCGcgUGGGGCU---CAUGU-CGU- -5' |
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| 24028 | 3' | -57.9 | NC_005262.1 | + | 44958 | 0.67 | 0.529552 |
|
Target: 5'- gGAGCAGauGGC-CGCCgaggcgagggCCGAcGUGCAGCAg -3' miRNA: 3'- -CUCGUU--CCGcGUGG----------GGCU-CAUGUCGU- -5' |
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| 24028 | 3' | -57.9 | NC_005262.1 | + | 53537 | 0.67 | 0.540066 |
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Target: 5'- cGAGUAuuucgccGCGUACCCCGAGaUGguGCu -3' miRNA: 3'- -CUCGUuc-----CGCGUGGGGCUC-AUguCGu -5' |
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| 24028 | 3' | -57.9 | NC_005262.1 | + | 30383 | 1.08 | 0.000648 |
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Target: 5'- cGAGCAAGGCGCACCCCGAGUACAGCAc -3' miRNA: 3'- -CUCGUUCCGCGUGGGGCUCAUGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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