Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24031 | 5' | -52.1 | NC_005262.1 | + | 31531 | 0.66 | 0.885935 |
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Target: 5'- cGCUUCAUc-GGCACGUCCGaaaacGCCGu -3' miRNA: 3'- -UGAAGUAcuCCGUGUAGGUacu--CGGU- -5' |
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| 24031 | 5' | -52.1 | NC_005262.1 | + | 42517 | 0.66 | 0.883635 |
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Target: 5'- uGCUUCGUGAuguugcggccggcuGGCGCcgCC-UGAaaGCCGg -3' miRNA: 3'- -UGAAGUACU--------------CCGUGuaGGuACU--CGGU- -5' |
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| 24031 | 5' | -52.1 | NC_005262.1 | + | 43623 | 0.66 | 0.853295 |
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Target: 5'- gACggCAgGcGGCACAUCCGguUGAGCUu -3' miRNA: 3'- -UGaaGUaCuCCGUGUAGGU--ACUCGGu -5' |
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| 24031 | 5' | -52.1 | NC_005262.1 | + | 49004 | 0.66 | 0.844501 |
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Target: 5'- cGCUUCAgGAGGU-CGUCC--GAGCCc -3' miRNA: 3'- -UGAAGUaCUCCGuGUAGGuaCUCGGu -5' |
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| 24031 | 5' | -52.1 | NC_005262.1 | + | 22318 | 0.67 | 0.816739 |
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Target: 5'- gGCcggCGUGGgcGGCGCggCCGUGAGCUu -3' miRNA: 3'- -UGaa-GUACU--CCGUGuaGGUACUCGGu -5' |
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| 24031 | 5' | -52.1 | NC_005262.1 | + | 38510 | 0.68 | 0.78713 |
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Target: 5'- gGCUcUCcgGGGGCACggCCGgcaaGAGCUAc -3' miRNA: 3'- -UGA-AGuaCUCCGUGuaGGUa---CUCGGU- -5' |
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| 24031 | 5' | -52.1 | NC_005262.1 | + | 19513 | 0.68 | 0.777933 |
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Target: 5'- cGCUUCAgaugccgcgacugcgGGGGCACAUUCAUccAGCCu -3' miRNA: 3'- -UGAAGUa--------------CUCCGUGUAGGUAc-UCGGu -5' |
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| 24031 | 5' | -52.1 | NC_005262.1 | + | 31413 | 1.09 | 0.002056 |
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Target: 5'- cACUUCAUGAGGCACAUCCAUGAGCCAa -3' miRNA: 3'- -UGAAGUACUCCGUGUAGGUACUCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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