Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24048 | 3' | -57.8 | NC_005262.1 | + | 46076 | 0.66 | 0.639537 |
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Target: 5'- uGACGUGCUCcgcauauuGGGAGG--ACGGccgcGCCg -3' miRNA: 3'- gUUGCACGAG--------UCCUCCcuUGCCc---CGG- -5' |
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| 24048 | 3' | -57.8 | NC_005262.1 | + | 50656 | 0.67 | 0.565115 |
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Target: 5'- gCAGCGgGCUCGGGcugcGGuuGAACGGGuGCg -3' miRNA: 3'- -GUUGCaCGAGUCCu---CC--CUUGCCC-CGg -5' |
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| 24048 | 3' | -57.8 | NC_005262.1 | + | 11301 | 0.67 | 0.544196 |
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Target: 5'- uGGCGUcgUCGGGAGGGA-CGGugaagaaaucGGCCc -3' miRNA: 3'- gUUGCAcgAGUCCUCCCUuGCC----------CCGG- -5' |
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| 24048 | 3' | -57.8 | NC_005262.1 | + | 38837 | 0.67 | 0.53383 |
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Target: 5'- -cGCGUGCUCAGGAaGGcACGGcagcGCUc -3' miRNA: 3'- guUGCACGAGUCCUcCCuUGCCc---CGG- -5' |
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| 24048 | 3' | -57.8 | NC_005262.1 | + | 14302 | 0.71 | 0.355683 |
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Target: 5'- -uGCGUGgaCGGGAGGGcGGCGGaaccGCCg -3' miRNA: 3'- guUGCACgaGUCCUCCC-UUGCCc---CGG- -5' |
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| 24048 | 3' | -57.8 | NC_005262.1 | + | 62433 | 0.71 | 0.339503 |
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Target: 5'- gCAACcgcgGUGCUacaaucgcacCGGGAaaauGGGAcuACGGGGCCg -3' miRNA: 3'- -GUUG----CACGA----------GUCCU----CCCU--UGCCCCGG- -5' |
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| 24048 | 3' | -57.8 | NC_005262.1 | + | 50082 | 0.72 | 0.308767 |
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Target: 5'- cCGGCGcgGCUUcaucGGcGGGAGCGGGcGCCg -3' miRNA: 3'- -GUUGCa-CGAGu---CCuCCCUUGCCC-CGG- -5' |
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| 24048 | 3' | -57.8 | NC_005262.1 | + | 49712 | 0.73 | 0.273395 |
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Target: 5'- aCGGCGUGC-CGGGcGGcGAAcCGGcGGCCg -3' miRNA: 3'- -GUUGCACGaGUCCuCC-CUU-GCC-CCGG- -5' |
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| 24048 | 3' | -57.8 | NC_005262.1 | + | 40281 | 1.12 | 0.000451 |
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Target: 5'- cCAACGUGCUCAGGAGGGAACGGGGCCg -3' miRNA: 3'- -GUUGCACGAGUCCUCCCUUGCCCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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