miRNA: 3'- aGUUGGg---GCCGCUGg---------CGAAGCUCGUa -5'
miRNA: 3'- -AGUUGGGGCCGCUg--GCGaaGC-UCGUa -5'
miRNA: 3'- aGUUGGGgCCGCUGGCGAAGCuCgua -5'
miRNA: 3'- aguUG-GGGCCGCUGGCGAAGCUCGUa -5'
miRNA: 3'- -AGUUGGGGCCGCUGGCGAAGCUCGUA- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24048 | 5' | -58.5 | NC_005262.1 | + | 55769 | 0.74 | 0.189092 |
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Target: 5'- cCAACCaguaCGGCGACaucguugcggGCUUCGAGCAg -3' miRNA: 3'- aGUUGGg---GCCGCUGg---------CGAAGCUCGUa -5' |
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| 24048 | 5' | -58.5 | NC_005262.1 | + | 27555 | 0.75 | 0.174719 |
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Target: 5'- cUCAGCCCCGGCGAggaCGCcgCGcAGCGc -3' miRNA: 3'- -AGUUGGGGCCGCUg--GCGaaGC-UCGUa -5' |
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| 24048 | 5' | -58.5 | NC_005262.1 | + | 9812 | 0.76 | 0.148868 |
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Target: 5'- cCAACUCgGGCGACCGCUUCGuGa-- -3' miRNA: 3'- aGUUGGGgCCGCUGGCGAAGCuCgua -5' |
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| 24048 | 5' | -58.5 | NC_005262.1 | + | 41197 | 0.9 | 0.014202 |
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Target: 5'- --cGCgCCCGGCGACCGCUUCGAGCAc -3' miRNA: 3'- aguUG-GGGCCGCUGGCGAAGCUCGUa -5' |
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| 24048 | 5' | -58.5 | NC_005262.1 | + | 40315 | 1.05 | 0.001004 |
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Target: 5'- uUCAACCCCGGCGACCGCUUCGAGCAUc -3' miRNA: 3'- -AGUUGGGGCCGCUGGCGAAGCUCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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