Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24053 | 3' | -51.1 | NC_005262.1 | + | 31846 | 0.66 | 0.952314 |
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Target: 5'- --uGCGGCGGAuguUGUGGAccgUGcGCUUGc -3' miRNA: 3'- ccuUGCCGCUUu--GCACCU---AC-CGAACc -5' |
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| 24053 | 3' | -51.1 | NC_005262.1 | + | 6116 | 0.66 | 0.952314 |
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Target: 5'- cGGAACGGCGGcacagucgccucGAUGUGcgcGGCcgGGa -3' miRNA: 3'- -CCUUGCCGCU------------UUGCACcuaCCGaaCC- -5' |
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| 24053 | 3' | -51.1 | NC_005262.1 | + | 36430 | 0.66 | 0.944987 |
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Target: 5'- uGGAUGGCacgGAAACGUGGucguucaagaagucUGGCgcaUGGg -3' miRNA: 3'- cCUUGCCG---CUUUGCACCu-------------ACCGa--ACC- -5' |
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| 24053 | 3' | -51.1 | NC_005262.1 | + | 16494 | 0.66 | 0.943046 |
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Target: 5'- cGAGCugGGCGAGGCGcUGGGcgcUGcGCUgGGg -3' miRNA: 3'- cCUUG--CCGCUUUGC-ACCU---AC-CGAaCC- -5' |
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| 24053 | 3' | -51.1 | NC_005262.1 | + | 46918 | 0.67 | 0.927078 |
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Target: 5'- cGAucCGGCGAAgGCGcaagccUGGAUGGaUUGGg -3' miRNA: 3'- cCUu-GCCGCUU-UGC------ACCUACCgAACC- -5' |
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| 24053 | 3' | -51.1 | NC_005262.1 | + | 31645 | 0.67 | 0.921197 |
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Target: 5'- --uACGGCGAugcccGCGUGGAaGGUaaGGa -3' miRNA: 3'- ccuUGCCGCUu----UGCACCUaCCGaaCC- -5' |
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| 24053 | 3' | -51.1 | NC_005262.1 | + | 5075 | 0.68 | 0.894905 |
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Target: 5'- -cGGCGGCGgcGCGUGcGAccGGCUcGGc -3' miRNA: 3'- ccUUGCCGCuuUGCAC-CUa-CCGAaCC- -5' |
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| 24053 | 3' | -51.1 | NC_005262.1 | + | 35518 | 0.68 | 0.887652 |
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Target: 5'- uGGGugGGCc--ACGUGGcagGGCUUcGGc -3' miRNA: 3'- -CCUugCCGcuuUGCACCua-CCGAA-CC- -5' |
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| 24053 | 3' | -51.1 | NC_005262.1 | + | 12701 | 0.69 | 0.85607 |
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Target: 5'- aGAGCGGauauuuCGUGGAUGGCg--- -3' miRNA: 3'- cCUUGCCgcuuu-GCACCUACCGaacc -5' |
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| 24053 | 3' | -51.1 | NC_005262.1 | + | 42108 | 1.15 | 0.001557 |
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Target: 5'- cGGAACGGCGAAACGUGGAUGGCUUGGg -3' miRNA: 3'- -CCUUGCCGCUUUGCACCUACCGAACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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