Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24054 | 3' | -49 | NC_005262.1 | + | 24463 | 0.74 | 0.671347 |
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Target: 5'- uCGAUGGcgcugcACGGCAcgagCGUCGCGCUUg -3' miRNA: 3'- -GCUACCuaa---UGUUGUa---GCAGCGCGAG- -5' |
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| 24054 | 3' | -49 | NC_005262.1 | + | 18052 | 0.74 | 0.660032 |
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Target: 5'- gCGA-GGAgUACGACAgucUCGUgaCGCGCUCg -3' miRNA: 3'- -GCUaCCUaAUGUUGU---AGCA--GCGCGAG- -5' |
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| 24054 | 3' | -49 | NC_005262.1 | + | 10292 | 0.75 | 0.591947 |
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Target: 5'- aGAcGGAUguucGCAACcgCGUCGcCGCUCg -3' miRNA: 3'- gCUaCCUAa---UGUUGuaGCAGC-GCGAG- -5' |
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| 24054 | 3' | -49 | NC_005262.1 | + | 62795 | 0.76 | 0.579542 |
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Target: 5'- gGGUGcgugcgcGAUUACc-CAUCGUCGCGCUCc -3' miRNA: 3'- gCUAC-------CUAAUGuuGUAGCAGCGCGAG- -5' |
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| 24054 | 3' | -49 | NC_005262.1 | + | 54540 | 0.76 | 0.557139 |
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Target: 5'- aGAauUGGAUUccggccaGCGACGUCGcCGCGUUCg -3' miRNA: 3'- gCU--ACCUAA-------UGUUGUAGCaGCGCGAG- -5' |
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| 24054 | 3' | -49 | NC_005262.1 | + | 42720 | 1.12 | 0.003337 |
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Target: 5'- aCGAUGGAUUACAACAUCGUCGCGCUCg -3' miRNA: 3'- -GCUACCUAAUGUUGUAGCAGCGCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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