miRNA: 3'- -GGaCGCgAUGUag--CGGa--UGCCGGUCU- -5'
miRNA: 3'- gGACGCGAUGU-AGCGG-AUG-CCGGucu -5'
miRNA: 3'- -GGACgcgAUGUA--GCGGAUGC-CGGUCu -5'
miRNA: 3'- gGACGCGaUGU------AGCGG--AUGCCGGUcu -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24065 | 3' | -56.3 | NC_005262.1 | + | 22234 | 0.67 | 0.626769 |
|
Target: 5'- cUCgGCGaUGCGggcaGCCgcgGCGGCCAGAc -3' miRNA: 3'- -GGaCGCgAUGUag--CGGa--UGCCGGUCU- -5' |
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| 24065 | 3' | -56.3 | NC_005262.1 | + | 58099 | 0.67 | 0.626769 |
|
Target: 5'- gCUGCGCUGUAcUCGCCcUGCuGGCCu-- -3' miRNA: 3'- gGACGCGAUGU-AGCGG-AUG-CCGGucu -5' |
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| 24065 | 3' | -56.3 | NC_005262.1 | + | 59904 | 0.67 | 0.615955 |
|
Target: 5'- aCCUGa--UACGUggCGCCUGCGgGCCGGu -3' miRNA: 3'- -GGACgcgAUGUA--GCGGAUGC-CGGUCu -5' |
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| 24065 | 3' | -56.3 | NC_005262.1 | + | 51604 | 0.66 | 0.712627 |
|
Target: 5'- gUUGCGC-ACGgucgucUCGCCguUGCGGCCGa- -3' miRNA: 3'- gGACGCGaUGU------AGCGG--AUGCCGGUcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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