miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
24070 3' -54.4 NC_005262.1 + 1245 0.67 0.72294
Target:  5'- aAGUGCUUCGGCgcUGAGCggauaGUUGCg- -3'
miRNA:   3'- cUCGCGAAGCCG--ACUUGga---CAACGac -5'
24070 3' -54.4 NC_005262.1 + 60570 0.67 0.690267
Target:  5'- cGGCGC-UCGGCgcgGucuACCUGUUcCUGa -3'
miRNA:   3'- cUCGCGaAGCCGa--Cu--UGGACAAcGAC- -5'
24070 3' -54.4 NC_005262.1 + 7739 0.68 0.634813
Target:  5'- cAGCGUga-GGCUGAGCUgggaaUGUUGCa- -3'
miRNA:   3'- cUCGCGaagCCGACUUGG-----ACAACGac -5'
24070 3' -54.4 NC_005262.1 + 36600 0.71 0.462316
Target:  5'- aAGCGCUUCGGCgcaaagggGAAUgCgguucgGUUGCUGg -3'
miRNA:   3'- cUCGCGAAGCCGa-------CUUG-Ga-----CAACGAC- -5'
24070 3' -54.4 NC_005262.1 + 50611 1.09 0.001105
Target:  5'- cGAGCGCUUCGGCUGAACCUGUUGCUGg -3'
miRNA:   3'- -CUCGCGAAGCCGACUUGGACAACGAC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.