Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24071 | 3' | -61.5 | NC_005262.1 | + | 8151 | 0.67 | 0.367871 |
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Target: 5'- uGCCGGAcGCGGccacgaGCGCGCggccCGGCuUCu -3' miRNA: 3'- gCGGCCUcUGCC------CGUGCGa---GCCGuAG- -5' |
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| 24071 | 3' | -61.5 | NC_005262.1 | + | 5692 | 0.66 | 0.437626 |
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Target: 5'- gGCCGGAGcggagGCaGGUGCGCUUuGCGUa -3' miRNA: 3'- gCGGCCUC-----UGcCCGUGCGAGcCGUAg -5' |
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| 24071 | 3' | -61.5 | NC_005262.1 | + | 5362 | 0.66 | 0.437626 |
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Target: 5'- uGCCGGGgcguccuuGACGGGCAgGgacgCGGCcguGUCg -3' miRNA: 3'- gCGGCCU--------CUGCCCGUgCga--GCCG---UAG- -5' |
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| 24071 | 3' | -61.5 | NC_005262.1 | + | 5183 | 0.67 | 0.393123 |
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Target: 5'- gCGUCGcGGGcGCGGGCGCGgC-CGGCGc- -3' miRNA: 3'- -GCGGC-CUC-UGCCCGUGC-GaGCCGUag -5' |
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| 24071 | 3' | -61.5 | NC_005262.1 | + | 5046 | 0.69 | 0.306141 |
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Target: 5'- gGCCGGcgccugaucGGCGGGCAgGCccgCGGCGg- -3' miRNA: 3'- gCGGCCu--------CUGCCCGUgCGa--GCCGUag -5' |
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| 24071 | 3' | -61.5 | NC_005262.1 | + | 4816 | 0.67 | 0.359704 |
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Target: 5'- gCGCgGGAGGCGugaGGC-CGC-CGGCGg- -3' miRNA: 3'- -GCGgCCUCUGC---CCGuGCGaGCCGUag -5' |
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| 24071 | 3' | -61.5 | NC_005262.1 | + | 4685 | 0.68 | 0.313404 |
|
Target: 5'- gGCCGGAu-CGGGC-CGC--GGCAUCc -3' miRNA: 3'- gCGGCCUcuGCCCGuGCGagCCGUAG- -5' |
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| 24071 | 3' | -61.5 | NC_005262.1 | + | 2951 | 0.72 | 0.196893 |
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Target: 5'- uCGCUcuauGGGGCGGGCacucACGCUgccCGGCAUCg -3' miRNA: 3'- -GCGGc---CUCUGCCCG----UGCGA---GCCGUAG- -5' |
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| 24071 | 3' | -61.5 | NC_005262.1 | + | 59 | 0.66 | 0.427587 |
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Target: 5'- uGCCGGGGAggcCGcGGCuCGCgaagaagUCGGCcgCg -3' miRNA: 3'- gCGGCCUCU---GC-CCGuGCG-------AGCCGuaG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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