Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24072 | 5' | -51.7 | NC_005262.1 | + | 59988 | 0.68 | 0.835068 |
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Target: 5'- cUUCCUCCGGGCCgucgaggaugGCCGGgUuccgACcGGCu -3' miRNA: 3'- -AAGGAGGCUUGGa---------UGGCUgA----UGuUCG- -5' |
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| 24072 | 5' | -51.7 | NC_005262.1 | + | 15964 | 0.68 | 0.7971 |
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Target: 5'- -gCUUCCGGucgACCUGCUGGCgaucgACGAGa -3' miRNA: 3'- aaGGAGGCU---UGGAUGGCUGa----UGUUCg -5' |
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| 24072 | 5' | -51.7 | NC_005262.1 | + | 15189 | 0.69 | 0.787131 |
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Target: 5'- cUCCUCguCGAGCUg--CGAUUGCAGGCc -3' miRNA: 3'- aAGGAG--GCUUGGaugGCUGAUGUUCG- -5' |
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| 24072 | 5' | -51.7 | NC_005262.1 | + | 41365 | 0.69 | 0.776995 |
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Target: 5'- -gCCUCCacGGGgUUGCCGGCgaGCAGGCg -3' miRNA: 3'- aaGGAGG--CUUgGAUGGCUGa-UGUUCG- -5' |
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| 24072 | 5' | -51.7 | NC_005262.1 | + | 50951 | 0.71 | 0.669041 |
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Target: 5'- -gCCgCCGAGCUgaaGCCGAC-GCAGGCc -3' miRNA: 3'- aaGGaGGCUUGGa--UGGCUGaUGUUCG- -5' |
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| 24072 | 5' | -51.7 | NC_005262.1 | + | 63063 | 0.73 | 0.546306 |
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Target: 5'- -aCCguUCCGAcgcauACCUGCUGGC-ACAAGCg -3' miRNA: 3'- aaGG--AGGCU-----UGGAUGGCUGaUGUUCG- -5' |
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| 24072 | 5' | -51.7 | NC_005262.1 | + | 57442 | 0.75 | 0.440449 |
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Target: 5'- aUCCgcgcgugUUCGAGCuCUGCCgcGACUACAAGCg -3' miRNA: 3'- aAGG-------AGGCUUG-GAUGG--CUGAUGUUCG- -5' |
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| 24072 | 5' | -51.7 | NC_005262.1 | + | 51657 | 1.11 | 0.001989 |
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Target: 5'- uUUCCUCCGAACCUACCGACUACAAGCa -3' miRNA: 3'- -AAGGAGGCUUGGAUGGCUGAUGUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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