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miRNA: 3'- cUGGUCga-GCCGU----UCGaCCG--ACGCGU- -5'
miRNA: 3'- cuGGUCGAGC-CGU---------------UCGa--CCGaCGCGU- -5'
miRNA: 3'- -CUGG---UCGa--GCCGu-UCGAc---CGACGCGU- -5'
miRNA: 3'- cUGgUCGaGCCGuUCGAcCGACGCGu -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 24073 | 5' | -59.9 | NC_005262.1 | + | 38539 | 0.66 | 0.473268 |
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Target: 5'- uGCCAGaggCGGCGcaggAGCcGGCguUGCGCGc -3' miRNA: 3'- cUGGUCga-GCCGU----UCGaCCG--ACGCGU- -5' |
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| 24073 | 5' | -59.9 | NC_005262.1 | + | 10836 | 0.66 | 0.457539 |
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Target: 5'- -cCCGGCUCGaGCAccuugccgccggcgaAGCccaGGCgGCGCAg -3' miRNA: 3'- cuGGUCGAGC-CGU---------------UCGa--CCGaCGCGU- -5' |
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| 24073 | 5' | -59.9 | NC_005262.1 | + | 8160 | 0.66 | 0.453651 |
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Target: 5'- cGGCCacgAGCgcgCGGCccGGCUucuuGCUGCGCAg -3' miRNA: 3'- -CUGG---UCGa--GCCGu-UCGAc---CGACGCGU- -5' |
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| 24073 | 5' | -59.9 | NC_005262.1 | + | 15762 | 0.66 | 0.473268 |
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Target: 5'- uGCgGGCgCGGCcGGCU-GCUGCGUg -3' miRNA: 3'- cUGgUCGaGCCGuUCGAcCGACGCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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