miRNA: 3'- cUGGUCGAGCCGU--UCGACCgaC-GCGU- -5'
miRNA: 3'- -CUGG-UCGAGC-CGUUCGacCGa---CGCGU- -5'
miRNA: 3'- cUGGUCGa---GCcGUUCGACCGaCGCGU- -5'
miRNA: 3'- -CUGGUCG------AGCCGUUcGACCG------------ACGCGu -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24073 | 5' | -59.9 | NC_005262.1 | + | 60740 | 0.75 | 0.113263 |
|
Target: 5'- cGCUGGCUCGGCGaaGGCUGGgaGaCGCGc -3' miRNA: 3'- cUGGUCGAGCCGU--UCGACCgaC-GCGU- -5' |
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| 24073 | 5' | -59.9 | NC_005262.1 | + | 60946 | 0.67 | 0.406635 |
|
Target: 5'- cGGCCaAGCUCGuGCGGGCacGCcagcGCGCAa -3' miRNA: 3'- -CUGG-UCGAGC-CGUUCGacCGa---CGCGU- -5' |
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| 24073 | 5' | -59.9 | NC_005262.1 | + | 62899 | 0.73 | 0.157172 |
|
Target: 5'- cGCCAGCgcgcCGaCGAGCUGGC-GCGCAa -3' miRNA: 3'- cUGGUCGa---GCcGUUCGACCGaCGCGU- -5' |
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| 24073 | 5' | -59.9 | NC_005262.1 | + | 63021 | 0.67 | 0.371298 |
|
Target: 5'- cGGCCAGUacggcaUCGGCAA-CUGGCgcaaguccagcaUGCGCc -3' miRNA: 3'- -CUGGUCG------AGCCGUUcGACCG------------ACGCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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