miRNA: 3'- -CUGGUCGAG--CC-GUUCGacCGACGCGU- -5'
miRNA: 3'- -CUGGUCGag--CCgUUC-GACCGaCGCGU- -5'
miRNA: 3'- cUGGUCG---AGCCGUuCGACCGa-CGCgu -5'
miRNA: 3'- -CUGGUCGA-GCCGuuCGACCgACgCGU- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24073 | 5' | -59.9 | NC_005262.1 | + | 4375 | 0.67 | 0.397604 |
|
Target: 5'- aGGCCAGCUUcaGGuCGAGC--GUUGCGCGu -3' miRNA: 3'- -CUGGUCGAG--CC-GUUCGacCGACGCGU- -5' |
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| 24073 | 5' | -59.9 | NC_005262.1 | + | 4156 | 0.69 | 0.307139 |
|
Target: 5'- cGACCAGCgcgaGGaGAGaCUGGC-GCGCGa -3' miRNA: 3'- -CUGGUCGag--CCgUUC-GACCGaCGCGU- -5' |
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| 24073 | 5' | -59.9 | NC_005262.1 | + | 898 | 0.69 | 0.307139 |
|
Target: 5'- cGCCAGCucgUCGGCGcGCUGGCggGUGa- -3' miRNA: 3'- cUGGUCG---AGCCGUuCGACCGa-CGCgu -5' |
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| 24073 | 5' | -59.9 | NC_005262.1 | + | 350 | 0.7 | 0.239327 |
|
Target: 5'- uGACCGGCUcCGGCu-GCUGGgUG-GCGa -3' miRNA: 3'- -CUGGUCGA-GCCGuuCGACCgACgCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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