miRNA: 3'- cUGGUCG-----------AGCCguUCGa----CCGaCGCGU- -5'
miRNA: 3'- cUGG----UCGAGCCGU----UCGa----CCGaCGCGU- -5'
miRNA: 3'- -CUGGUCGAGCCG--UUCGaCCGa-----CGCGU- -5'
miRNA: 3'- -CUGGUCGAGCCGUUCGACCGACGCGU- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24073 | 5' | -59.9 | NC_005262.1 | + | 45366 | 0.68 | 0.346204 |
|
Target: 5'- aACCAGCaauacagcaccUCGGguAGCaucugGGCgGCGCAg -3' miRNA: 3'- cUGGUCG-----------AGCCguUCGa----CCGaCGCGU- -5' |
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| 24073 | 5' | -59.9 | NC_005262.1 | + | 58627 | 0.68 | 0.338113 |
|
Target: 5'- cACCgucaGGCUCGGCAgucgAGCggccgGGC-GCGCGa -3' miRNA: 3'- cUGG----UCGAGCCGU----UCGa----CCGaCGCGU- -5' |
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| 24073 | 5' | -59.9 | NC_005262.1 | + | 24978 | 0.68 | 0.322348 |
|
Target: 5'- aGGCCGGCUUGGUcgAGGC-GGCauucgaGCGCGg -3' miRNA: 3'- -CUGGUCGAGCCG--UUCGaCCGa-----CGCGU- -5' |
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| 24073 | 5' | -59.9 | NC_005262.1 | + | 51745 | 1.08 | 0.000359 |
|
Target: 5'- cGACCAGCUCGGCAAGCUGGCUGCGCAg -3' miRNA: 3'- -CUGGUCGAGCCGUUCGACCGACGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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