miRNA: 3'- cuuUGCUaGC--UCCGGGaCAG--UCGGCU- -5'
miRNA: 3'- cUUUGCUAGC-------------UCCG-GGAcAGUCGGCU- -5'
miRNA: 3'- cuUUGCUAGCU------------CCGGgACagUCGGCU- -5'
miRNA: 3'- cuuUGCUAGCUCCGggaCAG------UCGGCU- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24075 | 5' | -55.1 | NC_005262.1 | + | 42149 | 0.66 | 0.760519 |
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Target: 5'- --cGCGG-CGccGGGCCCgGUCgcGGCCGAu -3' miRNA: 3'- cuuUGCUaGC--UCCGGGaCAG--UCGGCU- -5' |
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| 24075 | 5' | -55.1 | NC_005262.1 | + | 53661 | 0.66 | 0.769615 |
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Target: 5'- aAGGCGAUCGccaaccugcccgaGGGCaUCUaUCAGCCGGg -3' miRNA: 3'- cUUUGCUAGC-------------UCCG-GGAcAGUCGGCU- -5' |
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| 24075 | 5' | -55.1 | NC_005262.1 | + | 23445 | 0.66 | 0.780578 |
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Target: 5'- -cGACGAUCGAcaucagcgcgccGGCCgUG--AGCCGAu -3' miRNA: 3'- cuUUGCUAGCU------------CCGGgACagUCGGCU- -5' |
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| 24075 | 5' | -55.1 | NC_005262.1 | + | 5303 | 0.66 | 0.780578 |
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Target: 5'- --cGCGGUCGAGGCg--GUCggagcuGGCCGGc -3' miRNA: 3'- cuuUGCUAGCUCCGggaCAG------UCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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