Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24076 | 3' | -55.9 | NC_005262.1 | + | 45649 | 0.66 | 0.696136 |
Target: 5'- --cGGGCACGcgcucccGGuuGAGGCGcAGCGc -3' miRNA: 3'- guuCCCGUGCua-----CCugUUCCGC-UCGC- -5' |
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24076 | 3' | -55.9 | NC_005262.1 | + | 34125 | 0.66 | 0.706817 |
Target: 5'- --cGGGUugGgcGGGCugcgcGGGcGCGGGCGg -3' miRNA: 3'- guuCCCGugCuaCCUG-----UUC-CGCUCGC- -5' |
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24076 | 3' | -55.9 | NC_005262.1 | + | 43049 | 0.66 | 0.717426 |
Target: 5'- -uGGGGUuCGAccGGGCAGGGguCGAGCu -3' miRNA: 3'- guUCCCGuGCUa-CCUGUUCC--GCUCGc -5' |
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24076 | 3' | -55.9 | NC_005262.1 | + | 32296 | 0.66 | 0.738385 |
Target: 5'- --cGGGCGCGAacGuCGAGGC-AGCGc -3' miRNA: 3'- guuCCCGUGCUacCuGUUCCGcUCGC- -5' |
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24076 | 3' | -55.9 | NC_005262.1 | + | 44157 | 0.66 | 0.738385 |
Target: 5'- --cGcGCGCGAUcGGGCAGGaGCGcGGCGg -3' miRNA: 3'- guuCcCGUGCUA-CCUGUUC-CGC-UCGC- -5' |
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24076 | 3' | -55.9 | NC_005262.1 | + | 49149 | 0.66 | 0.727953 |
Target: 5'- ---cGGCACGGUGGGCGugcAGGCc-GCa -3' miRNA: 3'- guucCCGUGCUACCUGU---UCCGcuCGc -5' |
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24076 | 3' | -55.9 | NC_005262.1 | + | 17538 | 0.66 | 0.727953 |
Target: 5'- --cGGGCACGcgGucgcgcGCGAGGCG-GCa -3' miRNA: 3'- guuCCCGUGCuaCc-----UGUUCCGCuCGc -5' |
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24076 | 3' | -55.9 | NC_005262.1 | + | 10230 | 0.66 | 0.717426 |
Target: 5'- ----cGCGacgaccgauCGAUacaaGGACAAGGCGAGCGg -3' miRNA: 3'- guuccCGU---------GCUA----CCUGUUCCGCUCGC- -5' |
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24076 | 3' | -55.9 | NC_005262.1 | + | 60552 | 0.66 | 0.717426 |
Target: 5'- gCAAGGGCACGcu-GACGccGGCGcucGGCGc -3' miRNA: 3'- -GUUCCCGUGCuacCUGUu-CCGC---UCGC- -5' |
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24076 | 3' | -55.9 | NC_005262.1 | + | 982 | 0.66 | 0.706817 |
Target: 5'- cCGAGuGGCGaccUGcgGGGCGGGGCG-GUGu -3' miRNA: 3'- -GUUC-CCGU---GCuaCCUGUUCCGCuCGC- -5' |
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24076 | 3' | -55.9 | NC_005262.1 | + | 27544 | 0.66 | 0.716369 |
Target: 5'- aCGAGGGCgagcucagccccgGCGA-GGACGccGCGcAGCGc -3' miRNA: 3'- -GUUCCCG-------------UGCUaCCUGUucCGC-UCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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