Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24077 | 3' | -53.3 | NC_005262.1 | + | 50269 | 0.66 | 0.854713 |
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Target: 5'- --aCGAGGGCGcgcccGCGCCGGCcgagcccgugACGGc-- -3' miRNA: 3'- caaGUUCCUGU-----UGCGGCCG----------UGCCuuc -5' |
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| 24077 | 3' | -53.3 | NC_005262.1 | + | 5580 | 0.66 | 0.863027 |
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Target: 5'- aGUUCGugguacGGGCGGCGCCgcucuccgcaGGCgacGCGGAAa -3' miRNA: 3'- -CAAGUu-----CCUGUUGCGG----------CCG---UGCCUUc -5' |
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| 24077 | 3' | -53.3 | NC_005262.1 | + | 45701 | 0.66 | 0.863027 |
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Target: 5'- -aUCGAGGGcCGGCaGCCGGC-CGuGAu- -3' miRNA: 3'- caAGUUCCU-GUUG-CGGCCGuGC-CUuc -5' |
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| 24077 | 3' | -53.3 | NC_005262.1 | + | 14251 | 0.66 | 0.863027 |
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Target: 5'- ----cAGGACGcagcguCGCCGGagACGGAAGu -3' miRNA: 3'- caaguUCCUGUu-----GCGGCCg-UGCCUUC- -5' |
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| 24077 | 3' | -53.3 | NC_005262.1 | + | 30005 | 0.66 | 0.863027 |
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Target: 5'- --aCAAGGGCAGCGaagaUGGCAa-GAAGu -3' miRNA: 3'- caaGUUCCUGUUGCg---GCCGUgcCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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