Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24077 | 3' | -53.3 | NC_005262.1 | + | 48726 | 0.68 | 0.760164 |
Target: 5'- cGUUCuGGGACAACGUguCGGauguCGGGAa -3' miRNA: 3'- -CAAGuUCCUGUUGCG--GCCgu--GCCUUc -5' |
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24077 | 3' | -53.3 | NC_005262.1 | + | 29877 | 0.68 | 0.760164 |
Target: 5'- --aCGAGGA--GCGCgCGGCGCuucaGGAAGa -3' miRNA: 3'- caaGUUCCUguUGCG-GCCGUG----CCUUC- -5' |
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24077 | 3' | -53.3 | NC_005262.1 | + | 35533 | 0.68 | 0.758101 |
Target: 5'- --gCAGGGcuucggccaagccGCGAugucgcuCGCCGGCGCGGGAa -3' miRNA: 3'- caaGUUCC-------------UGUU-------GCGGCCGUGCCUUc -5' |
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24077 | 3' | -53.3 | NC_005262.1 | + | 50806 | 0.68 | 0.749796 |
Target: 5'- --cCGAGGGCAuggagcgcuucGCGCCGGagACGGGc- -3' miRNA: 3'- caaGUUCCUGU-----------UGCGGCCg-UGCCUuc -5' |
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24077 | 3' | -53.3 | NC_005262.1 | + | 53398 | 1.09 | 0.001961 |
Target: 5'- cGUUCAAGGACAACGCCGGCACGGAAGu -3' miRNA: 3'- -CAAGUUCCUGUUGCGGCCGUGCCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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