Results 41 - 42 of 42 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24080 | 3' | -54.9 | NC_005262.1 | + | 32970 | 0.66 | 0.783855 |
Target: 5'- uGCGcGUCGC-CGAGACGCgcacCGgguuuGCGAUGu -3' miRNA: 3'- -UGU-CAGCGaGUUCUGCGa---GC-----CGCUAC- -5' |
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24080 | 3' | -54.9 | NC_005262.1 | + | 22607 | 0.73 | 0.389744 |
Target: 5'- aGCGGUCGC-CGGGcgcgcACGCgaucgCGGCGGUGc -3' miRNA: 3'- -UGUCAGCGaGUUC-----UGCGa----GCCGCUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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