Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24081 | 3' | -59.7 | NC_005262.1 | + | 29418 | 0.69 | 0.393389 |
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Target: 5'- uUCGGCGA---GUUGGCGGugGUCUGc -3' miRNA: 3'- -GGCCGCUuccCGACCGUCugCAGGCu -5' |
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| 24081 | 3' | -59.7 | NC_005262.1 | + | 23813 | 0.69 | 0.368041 |
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Target: 5'- aUGuGCGAGGGGUUGGUcaucucauGGGCG-CCGGc -3' miRNA: 3'- gGC-CGCUUCCCGACCG--------UCUGCaGGCU- -5' |
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| 24081 | 3' | -59.7 | NC_005262.1 | + | 38953 | 0.69 | 0.359844 |
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Target: 5'- aCUGGCGcAGGGUgaucGGCAcGGCG-CCGGc -3' miRNA: 3'- -GGCCGCuUCCCGa---CCGU-CUGCaGGCU- -5' |
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| 24081 | 3' | -59.7 | NC_005262.1 | + | 17212 | 0.7 | 0.341479 |
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Target: 5'- gCGGCGAGgacGGcGCUGGCgucguggucgucguGGGCGUCgCGGg -3' miRNA: 3'- gGCCGCUU---CC-CGACCG--------------UCUGCAG-GCU- -5' |
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| 24081 | 3' | -59.7 | NC_005262.1 | + | 49281 | 0.71 | 0.306092 |
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Target: 5'- gCCGGa--AGuGCUGGC-GACGUCCGAg -3' miRNA: 3'- -GGCCgcuUCcCGACCGuCUGCAGGCU- -5' |
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| 24081 | 3' | -59.7 | NC_005262.1 | + | 60746 | 0.72 | 0.234426 |
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Target: 5'- cUCGGCGAA-GGCUGGgAGACGcgCuCGAa -3' miRNA: 3'- -GGCCGCUUcCCGACCgUCUGCa-G-GCU- -5' |
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| 24081 | 3' | -59.7 | NC_005262.1 | + | 55934 | 0.74 | 0.177394 |
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Target: 5'- uCCGGCGAucgAGGGC-GGCGGcCGUCaGAu -3' miRNA: 3'- -GGCCGCU---UCCCGaCCGUCuGCAGgCU- -5' |
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| 24081 | 3' | -59.7 | NC_005262.1 | + | 45785 | 0.77 | 0.107443 |
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Target: 5'- gCGGCGAAGGGC-GGCGG-CGUgCUGAu -3' miRNA: 3'- gGCCGCUUCCCGaCCGUCuGCA-GGCU- -5' |
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| 24081 | 3' | -59.7 | NC_005262.1 | + | 54364 | 1.1 | 0.000466 |
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Target: 5'- cCCGGCGAAGGGCUGGCAGACGUCCGAc -3' miRNA: 3'- -GGCCGCUUCCCGACCGUCUGCAGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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