Results 61 - 63 of 63 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24084 | 3' | -52.6 | NC_005262.1 | + | 61589 | 0.7 | 0.643988 |
Target: 5'- aCGCGGAcaagacgcucucGACCgagcAGAAGCGCGCgCGcCUGa -3' miRNA: 3'- -GCGCUU------------UUGG----UCUUCGCGCG-GUaGAC- -5' |
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24084 | 3' | -52.6 | NC_005262.1 | + | 61693 | 0.69 | 0.742484 |
Target: 5'- cCGUGAcGAUCAG-GGCGCGCCGg--- -3' miRNA: 3'- -GCGCUuUUGGUCuUCGCGCGGUagac -5' |
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24084 | 3' | -52.6 | NC_005262.1 | + | 63184 | 0.69 | 0.721094 |
Target: 5'- uGUGGGugcGCCAGAAGCaGCGCUAUg-- -3' miRNA: 3'- gCGCUUu--UGGUCUUCG-CGCGGUAgac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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