Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24086 | 5' | -52.5 | NC_005262.1 | + | 50390 | 0.66 | 0.858259 |
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Target: 5'- uUGuGCCcGCUcUCGCGGAGcUCguGGAUg -3' miRNA: 3'- -ACuCGG-CGA-AGCGCUUCaAGguCUUG- -5' |
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| 24086 | 5' | -52.5 | NC_005262.1 | + | 52306 | 0.66 | 0.858259 |
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Target: 5'- gGAGCUGCUgaccaaaGCGc---UCCGGAACa -3' miRNA: 3'- aCUCGGCGAag-----CGCuucaAGGUCUUG- -5' |
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| 24086 | 5' | -52.5 | NC_005262.1 | + | 22358 | 0.66 | 0.866565 |
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Target: 5'- aUGAuCCGCguggUGCGGAGggugUCCGGcGCg -3' miRNA: 3'- -ACUcGGCGaa--GCGCUUCa---AGGUCuUG- -5' |
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| 24086 | 5' | -52.5 | NC_005262.1 | + | 36776 | 0.66 | 0.874619 |
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Target: 5'- cGGGCUGCUUCGaucauGGUcauuccUCCAGuGCg -3' miRNA: 3'- aCUCGGCGAAGCgcu--UCA------AGGUCuUG- -5' |
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| 24086 | 5' | -52.5 | NC_005262.1 | + | 55640 | 0.66 | 0.874619 |
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Target: 5'- cGGGCCGCgcgcUCGUGGccgcgUCCGGcACg -3' miRNA: 3'- aCUCGGCGa---AGCGCUuca--AGGUCuUG- -5' |
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| 24086 | 5' | -52.5 | NC_005262.1 | + | 25695 | 0.66 | 0.874619 |
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Target: 5'- aGGGCCGCgaggGCGA-GUUCUAcAACa -3' miRNA: 3'- aCUCGGCGaag-CGCUuCAAGGUcUUG- -5' |
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| 24086 | 5' | -52.5 | NC_005262.1 | + | 14159 | 0.66 | 0.882413 |
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Target: 5'- -aGGCCGCcgaUCGCGAGG--CCAccGAGCg -3' miRNA: 3'- acUCGGCGa--AGCGCUUCaaGGU--CUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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