Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24086 | 5' | -52.5 | NC_005262.1 | + | 59366 | 0.73 | 0.50044 |
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Target: 5'- gGAGCCGCccCGUGAAGc-CCAGAAa -3' miRNA: 3'- aCUCGGCGaaGCGCUUCaaGGUCUUg -5' |
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| 24086 | 5' | -52.5 | NC_005262.1 | + | 43473 | 0.73 | 0.479535 |
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Target: 5'- cGAGCCGCUUCGUGgcGgcgaccgUCUAcgcGGACg -3' miRNA: 3'- aCUCGGCGAAGCGCuuCa------AGGU---CUUG- -5' |
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| 24086 | 5' | -52.5 | NC_005262.1 | + | 62414 | 0.74 | 0.429266 |
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Target: 5'- aGGGCCGCga-GUG-GGUUCCAGAAa -3' miRNA: 3'- aCUCGGCGaagCGCuUCAAGGUCUUg -5' |
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| 24086 | 5' | -52.5 | NC_005262.1 | + | 24355 | 0.76 | 0.322411 |
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Target: 5'- cGAGCUGCgUgGCGAGGUaaUCCgAGAGCa -3' miRNA: 3'- aCUCGGCGaAgCGCUUCA--AGG-UCUUG- -5' |
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| 24086 | 5' | -52.5 | NC_005262.1 | + | 26211 | 0.76 | 0.322411 |
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Target: 5'- cGAGCCGUacagcuacUUCGUGAAGUggcUCCAGGGg -3' miRNA: 3'- aCUCGGCG--------AAGCGCUUCA---AGGUCUUg -5' |
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| 24086 | 5' | -52.5 | NC_005262.1 | + | 42996 | 0.76 | 0.322411 |
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Target: 5'- cGGGCCGCUUCGUGucGggCCAGu-- -3' miRNA: 3'- aCUCGGCGAAGCGCuuCaaGGUCuug -5' |
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| 24086 | 5' | -52.5 | NC_005262.1 | + | 55831 | 1.11 | 0.001527 |
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Target: 5'- gUGAGCCGCUUCGCGAAGUUCCAGAACg -3' miRNA: 3'- -ACUCGGCGAAGCGCUUCAAGGUCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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