miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
24086 5' -52.5 NC_005262.1 + 52648 0.67 0.822674
Target:  5'- aGGGCCGCccgUGCGA----CCGGAGCc -3'
miRNA:   3'- aCUCGGCGaa-GCGCUucaaGGUCUUG- -5'
24086 5' -52.5 NC_005262.1 + 8267 0.67 0.822674
Target:  5'- --uGCUGCgUCGCGAAGUcgaguuUgCGGAACu -3'
miRNA:   3'- acuCGGCGaAGCGCUUCA------AgGUCUUG- -5'
24086 5' -52.5 NC_005262.1 + 41291 0.66 0.847096
Target:  5'- gGAcuGCCGCUUCGCGAuccgcucgcgcgccAGcUUCAGcGCu -3'
miRNA:   3'- aCU--CGGCGAAGCGCU--------------UCaAGGUCuUG- -5'
24086 5' -52.5 NC_005262.1 + 50390 0.66 0.858259
Target:  5'- uUGuGCCcGCUcUCGCGGAGcUCguGGAUg -3'
miRNA:   3'- -ACuCGG-CGA-AGCGCUUCaAGguCUUG- -5'
24086 5' -52.5 NC_005262.1 + 36776 0.66 0.874619
Target:  5'- cGGGCUGCUUCGaucauGGUcauuccUCCAGuGCg -3'
miRNA:   3'- aCUCGGCGAAGCgcu--UCA------AGGUCuUG- -5'
24086 5' -52.5 NC_005262.1 + 14159 0.66 0.882413
Target:  5'- -aGGCCGCcgaUCGCGAGG--CCAccGAGCg -3'
miRNA:   3'- acUCGGCGa--AGCGCUUCaaGGU--CUUG- -5'
24086 5' -52.5 NC_005262.1 + 24355 0.76 0.322411
Target:  5'- cGAGCUGCgUgGCGAGGUaaUCCgAGAGCa -3'
miRNA:   3'- aCUCGGCGaAgCGCUUCA--AGG-UCUUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.