Results 101 - 108 of 108 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24091 | 3' | -61.6 | NC_005262.1 | + | 59143 | 0.7 | 0.264129 |
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Target: 5'- cGGGCCG-CGUCGccGCGcGCaUGGAGgaCGCg -3' miRNA: 3'- -UCCGGCuGCAGCu-CGC-CG-ACCUC--GCG- -5' |
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| 24091 | 3' | -61.6 | NC_005262.1 | + | 59607 | 0.66 | 0.444638 |
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Target: 5'- cAGGCCGugGUCGuG-GGCgagaaGAaccucGCGCg -3' miRNA: 3'- -UCCGGCugCAGCuCgCCGac---CU-----CGCG- -5' |
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| 24091 | 3' | -61.6 | NC_005262.1 | + | 60343 | 0.66 | 0.463342 |
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Target: 5'- gAGGUCGACGggcgguUCGAGCuGauccccGAGCGCg -3' miRNA: 3'- -UCCGGCUGC------AGCUCGcCgac---CUCGCG- -5' |
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| 24091 | 3' | -61.6 | NC_005262.1 | + | 60391 | 0.68 | 0.311965 |
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Target: 5'- cGGCCGcaagauCGUCG-GCGGCau-GGCGCa -3' miRNA: 3'- uCCGGCu-----GCAGCuCGCCGaccUCGCG- -5' |
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| 24091 | 3' | -61.6 | NC_005262.1 | + | 60960 | 0.66 | 0.420971 |
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Target: 5'- cGGGCacgccagcgcgcaaaCGAgGUCGAgGCGcaGgUGGAGCGCc -3' miRNA: 3'- -UCCG---------------GCUgCAGCU-CGC--CgACCUCGCG- -5' |
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| 24091 | 3' | -61.6 | NC_005262.1 | + | 61148 | 0.68 | 0.35798 |
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Target: 5'- uGGCgcagcagaucgCGGCGgCGGGCGaGCUGacGGGCGCg -3' miRNA: 3'- uCCG-----------GCUGCaGCUCGC-CGAC--CUCGCG- -5' |
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| 24091 | 3' | -61.6 | NC_005262.1 | + | 61430 | 0.68 | 0.357176 |
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Target: 5'- cGGCCGAggaauuccaCGUCGcgaagaaGGCGGCggacGGcgaAGCGCu -3' miRNA: 3'- uCCGGCU---------GCAGC-------UCGCCGa---CC---UCGCG- -5' |
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| 24091 | 3' | -61.6 | NC_005262.1 | + | 62668 | 0.66 | 0.435447 |
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Target: 5'- gGGGUgGGuuuaGUCcgcccGGCGGCggcaggGGAGCGCg -3' miRNA: 3'- -UCCGgCUg---CAGc----UCGCCGa-----CCUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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