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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24093 | 5' | -56.4 | NC_005262.1 | + | 51585 | 0.75 | 0.203253 |
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Target: 5'- -aGCGCGCCGCCcAUCAGGgcgUGAAgGu -3' miRNA: 3'- ugCGCGCGGCGGuUAGUCC---ACUUgUu -5' |
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| 24093 | 5' | -56.4 | NC_005262.1 | + | 52158 | 0.72 | 0.299377 |
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Target: 5'- gACGCcgGCGCCGCCGAgccgauggugCAGG-GGGCAGa -3' miRNA: 3'- -UGCG--CGCGGCGGUUa---------GUCCaCUUGUU- -5' |
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| 24093 | 5' | -56.4 | NC_005262.1 | + | 53248 | 0.67 | 0.603891 |
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Target: 5'- cUGCGCGCgGCCGGUCGcGUcGGCAu -3' miRNA: 3'- uGCGCGCGgCGGUUAGUcCAcUUGUu -5' |
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| 24093 | 5' | -56.4 | NC_005262.1 | + | 57235 | 1.05 | 0.001368 |
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Target: 5'- gACGCGCGCCGCCAAUCAGGUGAACAAc -3' miRNA: 3'- -UGCGCGCGGCGGUUAGUCCACUUGUU- -5' |
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| 24093 | 5' | -56.4 | NC_005262.1 | + | 58731 | 0.68 | 0.500942 |
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Target: 5'- cACGCGcCGCCGCCGcgggccugcccgccgAUCAGGcgccggccggcGAGCAAc -3' miRNA: 3'- -UGCGC-GCGGCGGU---------------UAGUCCa----------CUUGUU- -5' |
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| 24093 | 5' | -56.4 | NC_005262.1 | + | 58898 | 0.66 | 0.636856 |
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Target: 5'- uGC-CGCGCUGCgCGAUCAGGccGGCGg -3' miRNA: 3'- -UGcGCGCGGCG-GUUAGUCCacUUGUu -5' |
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| 24093 | 5' | -56.4 | NC_005262.1 | + | 59591 | 0.66 | 0.658827 |
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Target: 5'- cGCGCGCGaCuaCGAUCAGGccguggucgUGGGCGAg -3' miRNA: 3'- -UGCGCGCgGcgGUUAGUCC---------ACUUGUU- -5' |
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| 24093 | 5' | -56.4 | NC_005262.1 | + | 59697 | 0.68 | 0.496802 |
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Target: 5'- -gGCGUGCCGCCAcgCGGGcgcuACGu -3' miRNA: 3'- ugCGCGCGGCGGUuaGUCCacu-UGUu -5' |
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| 24093 | 5' | -56.4 | NC_005262.1 | + | 62097 | 0.69 | 0.466268 |
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Target: 5'- cAUGCGuCGCCGCCGcggcaaccgcGUCGcGGaUGAACGAc -3' miRNA: 3'- -UGCGC-GCGGCGGU----------UAGU-CC-ACUUGUU- -5' |
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| 24093 | 5' | -56.4 | NC_005262.1 | + | 63323 | 0.66 | 0.647849 |
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Target: 5'- cACGCGCGCCGC--GUCgccgAGGUcAACGc -3' miRNA: 3'- -UGCGCGCGGCGguUAG----UCCAcUUGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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