Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24096 | 3' | -51.5 | NC_005262.1 | + | 25367 | 0.66 | 0.891865 |
Target: 5'- aACAgccgGU-GCGGCaUCAUCGCGCAGg -3' miRNA: 3'- -UGUaacaCAgCGUCG-AGUAGCGUGUU- -5' |
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24096 | 3' | -51.5 | NC_005262.1 | + | 57872 | 0.66 | 0.883459 |
Target: 5'- uACGccGUGUC-CAGCUCcugcgucgugaagAUCGCGCAAc -3' miRNA: 3'- -UGUaaCACAGcGUCGAG-------------UAGCGUGUU- -5' |
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24096 | 3' | -51.5 | NC_005262.1 | + | 25856 | 0.67 | 0.842048 |
Target: 5'- gGCGUUGgcGUCGCGGUcCAUgCGCGCc- -3' miRNA: 3'- -UGUAACa-CAGCGUCGaGUA-GCGUGuu -5' |
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24096 | 3' | -51.5 | NC_005262.1 | + | 37419 | 0.67 | 0.82344 |
Target: 5'- uGC-UUGgGUUGCAGCUCuAUCGCGuCGAc -3' miRNA: 3'- -UGuAACaCAGCGUCGAG-UAGCGU-GUU- -5' |
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24096 | 3' | -51.5 | NC_005262.1 | + | 36720 | 0.68 | 0.783703 |
Target: 5'- gGCGga-UGUCGCcgAGCUCG-CGCACGAa -3' miRNA: 3'- -UGUaacACAGCG--UCGAGUaGCGUGUU- -5' |
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24096 | 3' | -51.5 | NC_005262.1 | + | 58375 | 1.06 | 0.003221 |
Target: 5'- gACAUUGUGUCGCAGCUCAUCGCACAAa -3' miRNA: 3'- -UGUAACACAGCGUCGAGUAGCGUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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