miRNA: 3'- aCGA--CGUGCUGUg---------CCGGCGC-AGGGAc -5'
miRNA: 3'- aCGaCGUGCUGUGC-CGGcGCAGGGac -5'
miRNA: 3'- aCGa----CGUGCUGU-GCCGGCGC-AGgGac -5'
miRNA: 3'- aCGACG---UGCUGUG----------CCGGCGCaGGGAc -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24096 | 5' | -60.8 | NC_005262.1 | + | 50952 | 0.67 | 0.386954 |
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Target: 5'- aGCUcgGCACGACGguucccgcgaGGCCGCGcUCCUUc -3' miRNA: 3'- aCGA--CGUGCUGUg---------CCGGCGC-AGGGAc -5' |
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| 24096 | 5' | -60.8 | NC_005262.1 | + | 46314 | 0.67 | 0.378421 |
|
Target: 5'- cGC-GCGCGGCGCGcGCUuCGUCCUg- -3' miRNA: 3'- aCGaCGUGCUGUGC-CGGcGCAGGGac -5' |
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| 24096 | 5' | -60.8 | NC_005262.1 | + | 38963 | 0.68 | 0.370014 |
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Target: 5'- gGUgaucgGCACGGCGcCGGCCGCGaUCgCg- -3' miRNA: 3'- aCGa----CGUGCUGU-GCCGGCGC-AGgGac -5' |
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| 24096 | 5' | -60.8 | NC_005262.1 | + | 43073 | 0.66 | 0.478846 |
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Target: 5'- aGCUGCcgaGCGACGCgaccgugaugGGCUGCG-CCUUc -3' miRNA: 3'- aCGACG---UGCUGUG----------CCGGCGCaGGGAc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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