Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24097 | 5' | -57.2 | NC_005262.1 | + | 18575 | 0.66 | 0.609388 |
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Target: 5'- ---gGCGuUCAGG-CCGAGCCCgGCGg -3' miRNA: 3'- aagaUGCuAGUUCgGGCUCGGG-CGCa -5' |
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| 24097 | 5' | -57.2 | NC_005262.1 | + | 17383 | 0.66 | 0.620264 |
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Target: 5'- -cCUGCGGaccuugCGGGCCC-AGCCCGaCGa -3' miRNA: 3'- aaGAUGCUa-----GUUCGGGcUCGGGC-GCa -5' |
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| 24097 | 5' | -57.2 | NC_005262.1 | + | 41580 | 0.66 | 0.620264 |
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Target: 5'- ---cGCGGccgugagcuugUCGAGCgCGAGCCCgGCGUu -3' miRNA: 3'- aagaUGCU-----------AGUUCGgGCUCGGG-CGCA- -5' |
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| 24097 | 5' | -57.2 | NC_005262.1 | + | 58755 | 0.66 | 0.620264 |
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Target: 5'- -cCgcCGAUCAGGCgCCG-GCCgGCGa -3' miRNA: 3'- aaGauGCUAGUUCG-GGCuCGGgCGCa -5' |
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| 24097 | 5' | -57.2 | NC_005262.1 | + | 7525 | 0.66 | 0.642037 |
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Target: 5'- -aCUGCucgcCGAGCCCGAuGUCCGUGa -3' miRNA: 3'- aaGAUGcua-GUUCGGGCU-CGGGCGCa -5' |
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| 24097 | 5' | -57.2 | NC_005262.1 | + | 29646 | 0.66 | 0.642037 |
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Target: 5'- aUCggcaGCGAgcggCAugccGCCCGcGCCCGCGc -3' miRNA: 3'- aAGa---UGCUa---GUu---CGGGCuCGGGCGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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