Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24097 | 5' | -57.2 | NC_005262.1 | + | 50556 | 0.68 | 0.491768 |
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Target: 5'- -gCU-CGcgCAcccguucaaccgcAGCCCGAGCCCGCu- -3' miRNA: 3'- aaGAuGCuaGU-------------UCGGGCUCGGGCGca -5' |
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| 24097 | 5' | -57.2 | NC_005262.1 | + | 58665 | 1.04 | 0.001482 |
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Target: 5'- gUUCUACGAUCAAGCCCGAGCCCGCGUu -3' miRNA: 3'- -AAGAUGCUAGUUCGGGCUCGGGCGCA- -5' |
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| 24097 | 5' | -57.2 | NC_005262.1 | + | 58755 | 0.67 | 0.566164 |
|
Target: 5'- ---cACGAUCGAGCaguaCGAGCaggcacccuCCGCGUu -3' miRNA: 3'- aagaUGCUAGUUCGg---GCUCG---------GGCGCA- -5' |
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| 24097 | 5' | -57.2 | NC_005262.1 | + | 58755 | 0.66 | 0.620264 |
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Target: 5'- -cCgcCGAUCAGGCgCCG-GCCgGCGa -3' miRNA: 3'- aaGauGCUAGUUCG-GGCuCGGgCGCa -5' |
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| 24097 | 5' | -57.2 | NC_005262.1 | + | 58908 | 0.67 | 0.566164 |
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Target: 5'- ---cGCGAUCAGGCCgGcGGCCCGa-- -3' miRNA: 3'- aagaUGCUAGUUCGGgC-UCGGGCgca -5' |
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| 24097 | 5' | -57.2 | NC_005262.1 | + | 60048 | 0.67 | 0.576911 |
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Target: 5'- -gCU-CGAUC--GCCUGAGCCgCGCGg -3' miRNA: 3'- aaGAuGCUAGuuCGGGCUCGG-GCGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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