Results 41 - 47 of 47 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24098 | 3' | -62.9 | NC_005262.1 | + | 40854 | 0.72 | 0.14121 |
Target: 5'- aUCACGCCUCCCucgcgcggagcaugGCGUCGgcgagCGCGUa- -3' miRNA: 3'- gAGUGCGGAGGG--------------CGCGGCa----GCGCGaa -5' |
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24098 | 3' | -62.9 | NC_005262.1 | + | 46731 | 0.73 | 0.118295 |
Target: 5'- -gCGCGCCUaUCgGCGCCGUgCGCGCg- -3' miRNA: 3'- gaGUGCGGA-GGgCGCGGCA-GCGCGaa -5' |
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24098 | 3' | -62.9 | NC_005262.1 | + | 40559 | 0.73 | 0.116421 |
Target: 5'- -aCGCGCCgggcaacaccggcggUgCCGCGaCCGUCGCGCUg -3' miRNA: 3'- gaGUGCGG---------------AgGGCGC-GGCAGCGCGAa -5' |
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24098 | 3' | -62.9 | NC_005262.1 | + | 13468 | 0.73 | 0.124747 |
Target: 5'- --gGCGCgaagCCCGCGCgCGUCGCGCg- -3' miRNA: 3'- gagUGCGga--GGGCGCG-GCAGCGCGaa -5' |
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24098 | 3' | -62.9 | NC_005262.1 | + | 6173 | 0.75 | 0.092942 |
Target: 5'- gUCACGCgCUCCUGCGCC-UCGCGg-- -3' miRNA: 3'- gAGUGCG-GAGGGCGCGGcAGCGCgaa -5' |
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24098 | 3' | -62.9 | NC_005262.1 | + | 28234 | 0.75 | 0.090466 |
Target: 5'- uUCGCGCUcuuUCCCGCGCCGgcgagcgacaUCGCgGCUUg -3' miRNA: 3'- gAGUGCGG---AGGGCGCGGC----------AGCG-CGAA- -5' |
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24098 | 3' | -62.9 | NC_005262.1 | + | 58988 | 1.04 | 0.000486 |
Target: 5'- cCUCACGCCUCCCGCGCCGUCGCGCUUc -3' miRNA: 3'- -GAGUGCGGAGGGCGCGGCAGCGCGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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