Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24098 | 3' | -62.9 | NC_005262.1 | + | 50087 | 0.67 | 0.309046 |
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Target: 5'- --uGCGCCgaccgaaCCCGCGCCG-CGCGa-- -3' miRNA: 3'- gagUGCGGa------GGGCGCGGCaGCGCgaa -5' |
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| 24098 | 3' | -62.9 | NC_005262.1 | + | 50834 | 0.68 | 0.273794 |
|
Target: 5'- gUCgACGCCgagCCgGUcggcgauGCCGUCGCGCa- -3' miRNA: 3'- gAG-UGCGGa--GGgCG-------CGGCAGCGCGaa -5' |
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| 24098 | 3' | -62.9 | NC_005262.1 | + | 58607 | 0.7 | 0.194053 |
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Target: 5'- gUCGCGCCggCCGCGCC--CGCGCc- -3' miRNA: 3'- gAGUGCGGagGGCGCGGcaGCGCGaa -5' |
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| 24098 | 3' | -62.9 | NC_005262.1 | + | 58654 | 0.67 | 0.300451 |
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Target: 5'- -gCGCGCCgagcaggaucagCgCCGC-CCGUCGCGCa- -3' miRNA: 3'- gaGUGCGGa-----------G-GGCGcGGCAGCGCGaa -5' |
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| 24098 | 3' | -62.9 | NC_005262.1 | + | 58867 | 0.71 | 0.175197 |
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Target: 5'- gUCACGCCggcgcagCCCGCGCguuggcugcUGcCGCGCUg -3' miRNA: 3'- gAGUGCGGa------GGGCGCG---------GCaGCGCGAa -5' |
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| 24098 | 3' | -62.9 | NC_005262.1 | + | 58988 | 1.04 | 0.000486 |
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Target: 5'- cCUCACGCCUCCCGCGCCGUCGCGCUUc -3' miRNA: 3'- -GAGUGCGGAGGGCGCGGCAGCGCGAA- -5' |
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| 24098 | 3' | -62.9 | NC_005262.1 | + | 59216 | 0.66 | 0.374417 |
|
Target: 5'- gCUCACGCCcgagcagcgCCgccgcuggcgcgagaUGCGCCG-CGCGCg- -3' miRNA: 3'- -GAGUGCGGa--------GG---------------GCGCGGCaGCGCGaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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