Results 101 - 104 of 104 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
24103 | 5' | -56.6 | NC_005262.1 | + | 32561 | 0.66 | 0.71834 |
Target: 5'- cCGAcUUGGGCGCGUcgaucgacagGCGCgCGAUCUu -3' miRNA: 3'- -GCU-AGUCCGCGCGua--------CGUGgGCUAGG- -5' |
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24103 | 5' | -56.6 | NC_005262.1 | + | 47286 | 0.66 | 0.71834 |
Target: 5'- gGGUCAGccccaGCGCA-GCGCCCaGcgCCu -3' miRNA: 3'- gCUAGUCcg---CGCGUaCGUGGG-CuaGG- -5' |
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24103 | 5' | -56.6 | NC_005262.1 | + | 24727 | 0.66 | 0.71834 |
Target: 5'- uGAUCGcgcuGGCGCGCGcgaucggcgUGCGgCCGAa-- -3' miRNA: 3'- gCUAGU----CCGCGCGU---------ACGUgGGCUagg -5' |
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24103 | 5' | -56.6 | NC_005262.1 | + | 26174 | 0.66 | 0.71834 |
Target: 5'- aCGAgu-GGCGCGCG---AUCCGAUUCa -3' miRNA: 3'- -GCUaguCCGCGCGUacgUGGGCUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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