miRNA: 3'- -GCUAGUCCGCGCGUACGUGGGCUAGG- -5'
miRNA: 3'- -GCUAGU-CCGCGCGuaC---GUGGGCU-----AGG- -5'
miRNA: 3'- gCU--AG-UCCGCGCGUACgUG----GGC-UAGG- -5'
miRNA: 3'- -GCUAgUCc---GCGCGUaCGUGGG-CUAGg -5'
Results 101 - 104 of 104 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24103 | 5' | -56.6 | NC_005262.1 | + | 59976 | 1.11 | 0.000654 |
|
Target: 5'- uCGAUCAGGCGCGCAUGCACCCGAUCCu -3' miRNA: 3'- -GCUAGUCCGCGCGUACGUGGGCUAGG- -5' |
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| 24103 | 5' | -56.6 | NC_005262.1 | + | 61698 | 0.68 | 0.568991 |
|
Target: 5'- aCGAUCAgGGCGCGCcgGguuCGCUCGGcgcgcUCUg -3' miRNA: 3'- -GCUAGU-CCGCGCGuaC---GUGGGCU-----AGG- -5' |
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| 24103 | 5' | -56.6 | NC_005262.1 | + | 62109 | 0.7 | 0.437643 |
|
Target: 5'- uGAccUCaAGGCGCGCAUGgACugggCCGaAUCCa -3' miRNA: 3'- gCU--AG-UCCGCGCGUACgUG----GGC-UAGG- -5' |
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| 24103 | 5' | -56.6 | NC_005262.1 | + | 62708 | 0.69 | 0.516748 |
|
Target: 5'- aCGAUgGGuaauCGCGCAcGCACCCuGAUCg -3' miRNA: 3'- -GCUAgUCc---GCGCGUaCGUGGG-CUAGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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