miRNA: 3'- -GCua-GUCCG-CGCGUACG--------UGggCUAGg -5'
miRNA: 3'- -GCUAGUcc-GC-GCGUaCGUGGGCuAGG- -5'
miRNA: 3'- -GCUAGUCCgCGCGUACG------UGGGcUAGg -5'
miRNA: 3'- -GCUaGU-CCGCGCGUACG---------UG-GGCUaGG- -5'
Results 101 - 104 of 104 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24103 | 5' | -56.6 | NC_005262.1 | + | 1835 | 0.66 | 0.676086 |
|
Target: 5'- uCGccgCAGGCuGCGCAUGCgguugccuGCuuGGUCg -3' miRNA: 3'- -GCua-GUCCG-CGCGUACG--------UGggCUAGg -5' |
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| 24103 | 5' | -56.6 | NC_005262.1 | + | 1216 | 0.67 | 0.622444 |
|
Target: 5'- uCGAUCGauuCGuCGCAcGCACCCG-UCCg -3' miRNA: 3'- -GCUAGUcc-GC-GCGUaCGUGGGCuAGG- -5' |
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| 24103 | 5' | -56.6 | NC_005262.1 | + | 1081 | 0.69 | 0.537443 |
|
Target: 5'- cCGAUCAGG-GUGCGUGCgcgauuACCC-AUCg -3' miRNA: 3'- -GCUAGUCCgCGCGUACG------UGGGcUAGg -5' |
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| 24103 | 5' | -56.6 | NC_005262.1 | + | 481 | 0.66 | 0.686735 |
|
Target: 5'- gCGA-CGcGGCGCGCGUGUuccacgucgACgCCGAgCCa -3' miRNA: 3'- -GCUaGU-CCGCGCGUACG---------UG-GGCUaGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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