Results 101 - 104 of 104 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24103 | 5' | -56.6 | NC_005262.1 | + | 29901 | 0.66 | 0.665398 |
Target: 5'- cCGAUCGGGC-CGCcgGCcgacGCaCGAUCg -3' miRNA: 3'- -GCUAGUCCGcGCGuaCG----UGgGCUAGg -5' |
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24103 | 5' | -56.6 | NC_005262.1 | + | 34142 | 0.66 | 0.675019 |
Target: 5'- gCGGgcgCGGGCG-GCAUgccgcucGCugCCGAUgCCg -3' miRNA: 3'- -GCUa--GUCCGCgCGUA-------CGugGGCUA-GG- -5' |
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24103 | 5' | -56.6 | NC_005262.1 | + | 57924 | 0.66 | 0.675019 |
Target: 5'- uCGAUCGcuucauccgcacGGUGCugaccaGCGUGCAgaugaacCCCGAUCUg -3' miRNA: 3'- -GCUAGU------------CCGCG------CGUACGU-------GGGCUAGG- -5' |
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24103 | 5' | -56.6 | NC_005262.1 | + | 32561 | 0.66 | 0.71834 |
Target: 5'- cCGAcUUGGGCGCGUcgaucgacagGCGCgCGAUCUu -3' miRNA: 3'- -GCU-AGUCCGCGCGua--------CGUGgGCUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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