miRNA: 3'- -CGCUUCcAGCGGC---------CGGCGU---UCU--AGCa -5'
miRNA: 3'- -CGcUUCCagCGGCCGG-CGUUCUAGCa -5'
miRNA: 3'- -CGCUUCCAGCGGCCGGCGUUCUAGCA- -5'
miRNA: 3'- -CGCUUCCaGCgGCCGGCgUUCUagca -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24104 | 5' | -57.1 | NC_005262.1 | + | 58340 | 0.66 | 0.695905 |
|
Target: 5'- gGCGAGGuUCGCCGcgaggucauGCCGCGccgGGAcaUCGa -3' miRNA: 3'- -CGCUUCcAGCGGC---------CGGCGU---UCU--AGCa -5' |
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| 24104 | 5' | -57.1 | NC_005262.1 | + | 60203 | 0.73 | 0.302832 |
|
Target: 5'- gGC-AAGGa-GCCGGCCgGCGAGGUCGg -3' miRNA: 3'- -CGcUUCCagCGGCCGG-CGUUCUAGCa -5' |
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| 24104 | 5' | -57.1 | NC_005262.1 | + | 60379 | 1.08 | 0.000977 |
|
Target: 5'- gGCGAAGGUCGCCGGCCGCAAGAUCGUc -3' miRNA: 3'- -CGCUUCCAGCGGCCGGCGUUCUAGCA- -5' |
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| 24104 | 5' | -57.1 | NC_005262.1 | + | 61419 | 0.66 | 0.685336 |
|
Target: 5'- gGCGAAGG-CGgCGGCCG-AGGAa--- -3' miRNA: 3'- -CGCUUCCaGCgGCCGGCgUUCUagca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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