Results 101 - 108 of 108 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24105 | 3' | -64.8 | NC_005262.1 | + | 5366 | 0.68 | 0.249549 |
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Target: 5'- --gGGGCGuccUUGACGGGCagggaCGCGGCc -3' miRNA: 3'- ccgCCCGCuc-GACUGCCCGc----GCGCCG- -5' |
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| 24105 | 3' | -64.8 | NC_005262.1 | + | 39939 | 0.68 | 0.249549 |
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Target: 5'- cGGaCGGGCGgcaccGGCaagGGCGGcGUGC-CGGCc -3' miRNA: 3'- -CC-GCCCGC-----UCGa--CUGCC-CGCGcGCCG- -5' |
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| 24105 | 3' | -64.8 | NC_005262.1 | + | 46697 | 0.68 | 0.249549 |
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Target: 5'- cGGUGGaccuccucGCGGcccgccGCgucGACGGGCGCGCcuaucGGCg -3' miRNA: 3'- -CCGCC--------CGCU------CGa--CUGCCCGCGCG-----CCG- -5' |
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| 24105 | 3' | -64.8 | NC_005262.1 | + | 40247 | 0.68 | 0.255557 |
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Target: 5'- cGGCGGGCG-GUUGGucaGucUGCGCGGUc -3' miRNA: 3'- -CCGCCCGCuCGACUg--CccGCGCGCCG- -5' |
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| 24105 | 3' | -64.8 | NC_005262.1 | + | 62319 | 0.68 | 0.255557 |
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Target: 5'- aGCGGcaGCGccGGCaGACGaGCGgGCGGCg -3' miRNA: 3'- cCGCC--CGC--UCGaCUGCcCGCgCGCCG- -5' |
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| 24105 | 3' | -64.8 | NC_005262.1 | + | 29643 | 0.67 | 0.267297 |
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Target: 5'- uGCcGGCGAcgGCcGGCGGGUagacguagaagauGCGCGGUg -3' miRNA: 3'- cCGcCCGCU--CGaCUGCCCG-------------CGCGCCG- -5' |
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| 24105 | 3' | -64.8 | NC_005262.1 | + | 917 | 0.67 | 0.280777 |
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Target: 5'- uGGCGGGUGAuGgUGucgcccaucuucACGGGCGUGCc-- -3' miRNA: 3'- -CCGCCCGCU-CgAC------------UGCCCGCGCGccg -5' |
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| 24105 | 3' | -64.8 | NC_005262.1 | + | 30859 | 0.66 | 0.337051 |
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Target: 5'- cGGuCGGaGCGAucgugaaggcGCUGGCGaGcGuCGCGCGuGCg -3' miRNA: 3'- -CC-GCC-CGCU----------CGACUGC-C-C-GCGCGC-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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