Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24107 | 3' | -56.7 | NC_005262.1 | + | 31888 | 0.67 | 0.642678 |
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Target: 5'- cGUC-CGGCGCgUCGAGcggguUGCCCGcgUAGc -3' miRNA: 3'- -CGGaGCCGCG-AGCUC-----AUGGGCuuGUC- -5' |
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| 24107 | 3' | -56.7 | NC_005262.1 | + | 29565 | 0.67 | 0.621255 |
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Target: 5'- -aCUCGGCGCccaugUCGAGcgugucGCCCGAagccgcgaucGCGGg -3' miRNA: 3'- cgGAGCCGCG-----AGCUCa-----UGGGCU----------UGUC- -5' |
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| 24107 | 3' | -56.7 | NC_005262.1 | + | 59206 | 0.67 | 0.621255 |
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Target: 5'- uCCUgCgGGCGCUCac--GCCCGAGCAGc -3' miRNA: 3'- cGGA-G-CCGCGAGcucaUGGGCUUGUC- -5' |
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| 24107 | 3' | -56.7 | NC_005262.1 | + | 46026 | 0.67 | 0.621255 |
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Target: 5'- uGCgCUCGGC-CUCGA--AUUCGAGCAGc -3' miRNA: 3'- -CG-GAGCCGcGAGCUcaUGGGCUUGUC- -5' |
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| 24107 | 3' | -56.7 | NC_005262.1 | + | 22709 | 0.67 | 0.610553 |
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Target: 5'- gGCCggCGGCGUUgaCGAgcGUGCCgGGACAc -3' miRNA: 3'- -CGGa-GCCGCGA--GCU--CAUGGgCUUGUc -5' |
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| 24107 | 3' | -56.7 | NC_005262.1 | + | 38889 | 0.68 | 0.599869 |
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Target: 5'- uGCCUCGGCGUccUCcGGcUGgCCGAGCu- -3' miRNA: 3'- -CGGAGCCGCG--AGcUC-AUgGGCUUGuc -5' |
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| 24107 | 3' | -56.7 | NC_005262.1 | + | 9039 | 0.68 | 0.599869 |
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Target: 5'- cGCCUCGGCG-UCGAcGUGcguuuucccuuCCUGAucGCGGa -3' miRNA: 3'- -CGGAGCCGCgAGCU-CAU-----------GGGCU--UGUC- -5' |
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| 24107 | 3' | -56.7 | NC_005262.1 | + | 30502 | 0.68 | 0.599869 |
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Target: 5'- gGCUUCGGCGCcCGGcGUGCgCGcgauGCGGu -3' miRNA: 3'- -CGGAGCCGCGaGCU-CAUGgGCu---UGUC- -5' |
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| 24107 | 3' | -56.7 | NC_005262.1 | + | 4685 | 0.79 | 0.122063 |
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Target: 5'- cGCCUCGGCGCgguaguagUCGuAGUugCCGAGCu- -3' miRNA: 3'- -CGGAGCCGCG--------AGC-UCAugGGCUUGuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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